Aislamiento y cultivo de células epiteliales mamarias humanas primarias

2.5K visualizaciones

05:05 min.

3 de mayo de 2024

2.5K visualizaciones

Linfeng Qian^*¹ , Jiaxi Yan^*¹ , Shiqi Chen¹ , Maryam Mohanmmed Abbas Karekad² , Yue Wu³^,⁴ , Xunwei Wu⁵ , Xiaohong Xu³^,⁴ , Xiaoqun Zhang⁶^,⁷

¹The First Clinical Medical College of Zhejiang Chinese Medical University, ²The International Education College of Zhejiang Chinese Medical University, ³Department of Breast Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁴Bozhou District Hospital of Traditional Chinese Medicine, ⁵Engineering Laboratory for Biomaterials and Tissue Regeneration, Ningbo Stomatology Hospital, Ningbo, China and Savaid Stomatology School, Hangzhou Medical College, ⁶Department of Surgery, Kaihua District, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), ⁷Kaihua County Hospital of Traditional Chinese Medicine

^* These authors contributed equally

El presente estudio informa de un protocolo más fácil, económico y que ahorra tiempo para aislar y cultivar de manera eficiente células epiteliales mamarias humanas primarias (HMEC) a partir de pequeñas cantidades de tejido mamario. Este protocolo es adecuado para producir rápidamente HMEC primarios tanto para aplicaciones de laboratorio como clínicas.