Name: Método de muestreo de sangre a través de la pared para minimizar la interrupción del sueño en entornos clínicos (Video) | JoVE Journal
Uploaded: 2025-06-13T14:00:00.000+00:00
Duration: 6 min 39 s
Description: Aprenda el método de muestreo de sangre a través de la pared para investigar ritmos circadianos y salud cardiometabólica sin interrumpir el sueño. Esta técnica permite monitorear marcadores metabólicos durante 24 horas de forma continua.

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método de muestreo de sangre a través de la pared para minimizar la interrupción del sueño en entornos clínicos

Through-the-Wall Blood Sampling Method to Minimize Sleep Disruption in Clinical Settings

Método de muestreo de sangre a través de la pared para minimizar la interrupción del sueño en entornos clínicos

Physiological rhythms, including diurnal and circadian rhythms, play a critical role in health, with disturbances increasingly linked to cardiovascular ...

through-wall-blood-sampling-method-to-minimize-sleep-disruption

Research

Investigación

JoVE Journal

Medicine

Medicina

Cateterización crónica de los vasos sanguíneos de la rata es a menudo necesaria para la administración de sustancias, obtener muestras de sangre durante un período de tiempo o por mediciones directas consciente de la presión arterial. Cateterización arterial femoral de la rata y las mediciones correspondientes de la presión arterial en el animal consciente se ha demostrado.

El cateterismo femoral arterial y venosa para muestras de sangre, la Administración de Drogas y la presión arterial consciente y mediciones del ritmo cardíaco

Describimos una metodología para el procesamiento de sangre total para obtener una variedad de componentes para su posterior análisis. Hemos optimizado un protocolo simplificado que permite el procesamiento simultáneo rápido y de alto rendimiento de muestras de sangre completa en un entorno no clínico.

Fraccionamiento rápida y aislamiento de componentes de sangre total en las muestras obtenidas a partir de una configuración basada en la comunidad

Aquí, presentamos un protocolo para mejorar la calidad de los datos de sueño Inicio pruebas proporciona un método para mejorar las instrucciones mediante una visita estructurada participante. Este protocolo incluye la implementación de un manual educativo paso a paso con fotos para asegurar la colocación adecuada del equipo.

Sueño hogar multimodal en adultos mayores

La pérdida de sueño y la desalineación circadiana contribuyen a numerosos accidentes e incidentes operativos. La eficacia de las contramedidas y los diseños de programación de trabajo destinados a mitigar la fatiga puede ser difícil de evaluar en entornos operativos. Este manuscrito resume un enfoque para recopilar datos de sueño, circadianos, fatiga y rendimiento en entornos operativos complejos.

Recopilación de datos de sueño, circadiano, fatiga y rendimiento en entornos operativos complejos

Aquí, presentamos un método para investigar los ritmos diurnos en el rendimiento después de la categorización precisa de los participantes en grupos de fenotipos circadianos basados en el Cuestionario ChronoType de Múnich, biomarcadores de fase circadiana estándar de oro y medidas actigráficas.

Fenotipado circadiano humano y pruebas de rendimiento diurno en el mundo real

El presente protocolo describe un método simple y eficiente para recolectar sangre de la vena subclavia en ratas. Permite un muestreo rápido, oportuno y fácilmente identificable sin anestesia y obtiene sangre de alta calidad a través de la recolección repetitiva de muestras.

Muestreo repetitivo de sangre de la vena subclavia de rata consciente

El presente protocolo describe un método simple y eficiente para la recolección dinámica y en tiempo real de sangre de corazón de rata utilizando la técnica de microdiálisis.

Extracción dinámica continua de sangre del corazón de rata mediante una técnica de microdiálisis no invasiva

El protocolo muestra repetidas recolecciones de líquido cefalorraquídeo y sangre de ratas epilépticas realizadas en paralelo con un monitoreo continuo de video-electroencefalograma (EEG). Estos son fundamentales para explorar posibles vínculos entre los cambios en varias moléculas de fluidos corporales y la actividad convulsiva.

Toma de muestras de líquido cefalorraquídeo y sangre de la vena lateral de la cola en ratas durante los registros de EEG

La pulsatilidad de la hormona luteinizante (LH) es un sello distintivo de la función reproductiva. Describimos un protocolo para la activación remota de poblaciones neuronales específicas vinculadas a la extracción automatizada de sangre en serie. Esta técnica permite la modulación hormonal cronometrada, la multiplexación y la minimización de los efectos de manipulación sobre los niveles de LH en animales conscientes que se mueven libremente y no son molestados.

Activación neuronal remota junto con muestreo de sangre automatizado para inducir y medir la hormona luteinizante circulante en ratones

Aquí presentamos una combinación de métodos efectivos de restricción en ratas y punción de la vena subclavia que permiten la recolección de sangre rápida, segura y repetida en ratas sin anestesia.

Muestreo de sangre de la vena subclavia en ratas conscientes

Aprenda el método de muestreo de sangre a través de la pared para investigar ritmos circadianos y salud cardiometabólica sin interrumpir el sueño. Esta técnica permite monitorear marcadores metabólicos durante 24 horas de forma continua.

Método de muestreo de sangre a través de la pared para minimizar la interrupción del sueño en entornos clínicos

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