Name: Marcaje de isótopos estables in vivo para la identificación por espectrometría de masas de metabolitos paternos transferidos del espermatozoide al ovocito durante la fertilización (Video) | JoVE Journal
Uploaded: 2025-06-17T14:00:00.000+00:00
Duration: 5 min 55 s
Description: Aprende a identificar metabolitos paternos transferidos al cigoto durante la fertilización mediante marcaje de isótopos estables y espectrometría de masas. Este método revela el papel paterno en la herencia epigenética.

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<table><tbody><tr><td>2H2O filtrado, 99,8 % atómico 2H</td><td>Sigma Aldrich</td><td>756822</td><td></td></tr><tr><td>Acetona</td><td>Sigma Aldrich</td><td>179124</td><td></td></tr><tr><td>Agilent&nbsp; SIL-8</td><td>Agilent (Santa Clara, CA, EE. UU.)</td><td></td><td></td></tr><tr><td>C16-Ceramida-13C3-15N</td><td>Estándar LGC</td><td>TRC-C342770-25MG</td><td></td></tr><tr><td>diclorometano</td><td>Sigma Aldrich</td><td>34856</td><td></td></tr><tr><td>Éter dietílico&nbsp;</td><td>Sigma Aldrich</td><td>673811</td><td></td></tr><tr><td>etanol</td><td>Sigma Aldrich</td><td>1,11,727</td><td></td></tr><tr><td>HCG (gonadotropina coriónica humana,</td><td>Sigma Aldrich</td><td>CG5-1VL</td><td></td></tr><tr><td>Clorhidrato de L-lisina-&épsilon;-15N</td><td>Sigma Aldrich</td><td>608971</td><td></td></tr><tr><td>Hombres y mujeres&nbsp; Ratones C57BL/6JOlaHsd</td><td>Inotiv&nbsp;</td><td>C57BL/6JOlaHsd</td><td></td></tr><tr><td>metil terc-butil éter (MTBE)</td><td>Sigma Aldrich</td><td>306975</td><td></td></tr><tr><td>MetOH</td><td>Sigma Aldrich</td><td>1,06,035</td><td></td></tr><tr><td>N,O-Bis(trimetilsilil(TMS) trifluoroacetamida (BSTFA)</td><td>Supelco</td><td>15238</td><td></td></tr><tr><td>PBS</td><td>Aplicar</td><td>A0965-9010</td><td></td></tr><tr><td>PMSG (yegua preñada) s suero)</td><td>Lee Biosolutions</td><td>493-10</td><td></td></tr><tr><td>piridina</td><td>Sigma Aldrich</td><td>360570</td><td></td></tr><tr><td>Shimadzu GCMS 2010plus&nbsp;</td><td>Shimadzu</td><td></td><td></td></tr><tr><td>Sitostanol-5,6,22,23-d4</td><td>Sigma Aldrich</td><td>680028</td><td></td></tr><tr><td>trifluoracetato de sodio</td><td>Sigma Aldrich</td><td>1615138</td><td></td></tr><tr><td>SolariX XR 7T&nbsp;</td><td>Bruker Daltonics</td><td></td><td></td></tr><tr><td>TimsTOF Pro Q-TOF&nbsp;</td><td>Bruker Daltonics</td><td></td><td></td></tr><tr><td>U13C glucosa&nbsp;</td><td>Sigma Aldrich</td><td>389374</td><td></td></tr><tr><td>Ultimate RS3000 UHPLC&nbsp;</td><td>Thermo Fisher Scientific</td><td></td><td></td></tr><tr><td>Precolumna VanGuard CSHTM (5,0 &tiempos; 2,1 mm; 1,7 &mu; m, 130 &Aring;)</td><td>Aguas</td><td></td><td></td></tr></tbody></table>

marcaje de isótopos estables in vivo para la identificación por espectrometría de masas de metabolitos paternos transferidos del espermatozoide al ovocito durante la fertilización

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Marcaje de isótopos estables in vivo para la identificación por espectrometría de masas de metabolitos paternos transferidos del espermatozoide al ovocito durante la fertilización

Paternal contribution to embryo genetics has so far been limited to allelic sequences for decades. A decade of results suggests, instead, that epigenetic ...

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Marcaje de isótopos estables in vivo para la identificación por espectrometría de masas de metabolitos paternos transferidos del espermatozoide al ovocito durante la fertilización (Video) | JoVE Journal

Marcaje de isótopos estables in vivo para la identificación por espectrometría de masas de metabolitos paternos transferidos del espermatozoide al ovocito durante la fertilización

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