Name: Elastómeros magnetorreológicos micromodelados para impulsar cambios en la alineación de los cardiomiocitos (Video) | JoVE Journal
Uploaded: 2025-06-10T12:40:00.000+00:00
Duration: 8 min 10 s
Description: Aprende a fabricar elastómeros magnetorreológicos micromodelados para estudiar la alineación de cardiomiocitos en respuesta a cambios dinámicos de rigidez. Esta plataforma permite investigar la memoria mecánica cardíaca.

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</ol>

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elastómeros magnetorreológicos micromodelados para impulsar cambios en la alineación de los cardiomiocitos

Micropatterned Magneto-Rheological Elastomers to Drive Changes in Cardiomyocyte Alignment

Elastómeros magnetorreológicos micromodelados para impulsar cambios en la alineación de los cardiomiocitos

Substrate-associated cues, such as mechanical and topographic, profoundly influence cellular response. However, much of the foundational research employs ...

micropatterned-magneto-rheological-elastomers-to-drive-changes

Research

Investigación

JoVE Journal

Bioengineering

Bioingeniería

En este protocolo, demostramos la fabricación de sustratos biomiméticos de cultivo de células cardíacas hechos de dos materiales poliméricos distintos utilizando litografía de fuerza capilar.  Los métodos descritos proporcionan una técnica escalable y rentable para diseñar la estructura y función de los tejidos cardíacos macroscópicos para aplicaciones in vitro e in vivo.

Capilar Fuerza Litografía Cardiaca de Ingeniería de Tejidos

La generación de tejido alineado de miocardio es un requisito clave para la adaptación de los recientes avances en la biología de células madre para fines de utilidad clínica. Aquí se describe un enfoque para la impresión por microcontacto el control preciso de la forma y la función celular. Usando poblaciones altamente purificadas de células madre embrionarias derivadas progenitores cardíacos, a continuación, generar anisotrópico tejido miocárdico funcional.

Generación de tejido funcional del miocardio Alineados través de la impresión por microcontacto

Presentamos un nuevo hidrogel de poliacrilamida, llamado hidroxi-Paam, que permite una unión directa de las proteínas ECM con costo o experiencia mínima. La combinación de hidroxi-Paam hidrogeles con impresión por microcontacto facilita el control independiente de muchas señales del microambiente celular natural para el estudio de mechanostransduction celular.

Preparación de hidroxi-Paam hidrogeles para desacoplar los efectos de mecanotransducción Cues

Este protocolo describe un método novedoso para crear un modelo de elementos finitos espacial detallada de la arquitectura intracelular de cardiomiocitos de imágenes de microscopía confocal y microscopia electrónica. La potencia de este modelo espacial detallada se demuestra mediante estudios de caso en la señalización de calcio y bioenergética.

Creando un modelo geométrico realista estructural de elementos finitos de cardiomiocitos para estudiar el papel de la arquitectura celular de cardiomiocitos biología de sistemas

Aquí presentamos un protocolo para la formación de una población celular mediante estímulos eléctricos y mecánicos, emulando la fisiología cardiaca. Esta estimulación electromecánica realza el potencial cardiomiogenético de las células tratadas y es una estrategia prometedora para la terapia celular más, modelado de la enfermedad y drogas.

Simultánea estimulación eléctrica y mecánica para mejorar el potencial de las células cardiomiogenético

Un andamio bioinspirado se fabrica mediante una técnica de fotolitografía suave utilizando hidrogeles mecánicamente robustos y conductores eléctricos. Los hidrogeles micropatrónizados proporcionan alineación direccional de células de cardiomiocitos, lo que resulta en una dirección personalizada de accionamiento. Los microelectrodos flexibles también se integran en el andamio para lograr control eléctrico para un tejido cardíaco autoaccionado.

Robot suave bioinspirado con microelectrodos incorporados

Este protocolo proporciona métodos detallados que describen la fabricación e implementación de una plataforma de ajuste de la carga posterior basada en magnéticos para tejidos cardíacos diseñados.

Ajuste magnético de la carga posterior en los tejidos cardíacos diseñados

Este protocolo introduce un método de micropatrón libre de litografía que es simple y accesible para aquellos con un fondo de bioingeniería limitado. Este método utiliza plantillas de corte láser personalizadas para proteínas de matriz extracelular micropatrón en una forma de interés para la modulación de morfologías celulares. El procedimiento para el micropatrón se demuestra utilizando cardiomiocitos derivados de células madre pluripotentes inducidas.

Micropatrón simple de células únicas sin litografía con plantillas de corte láser

El presente protocolo genera tejidos cardíacos de ingeniería en forma de malla que contienen células cardiovasculares derivadas de células madre pluripotentes inducidas por el hombre para permitir la investigación de la terapia de implantación celular para enfermedades del corazón.

Preparación de tejidos cardíacos de ingeniería en forma de malla derivados de células iPS humanas para la reparación del miocardio In Vivo

Este protocolo tiene como objetivo describir una nueva metodología para medir la velocidad de disparo cardíaco intrínseca utilizando el registro de la matriz de microelectrodos de todo el tejido del ganglio sinoauricular para identificar defectos de marcapasos en ratones. Los agentes farmacológicos también se pueden introducir en este método para estudiar sus efectos sobre el marcapasos intrínseco.

Registro de la matriz de microelectrodos de la tasa de disparo del nódulo sinoauricular para identificar defectos intrínsecos de marcapasos cardíacos en ratones

Este trabajo presenta un protocolo flexible para utilizar la tecnología de superficies contráctiles elastoméricas marcadas fluorescentemente (FLECS) en formato de micropozos para una cuantificación simplificada y sin intervención de las fuerzas contráctiles de una sola célula basada en desplazamientos visualizados de micropatrones de proteínas fluorescentes.

Análisis simplificado y de alto rendimiento de la contractilidad de una sola célula utilizando elastómeros con micropatrones

Aprende a fabricar elastómeros magnetorreológicos micromodelados para estudiar la alineación de cardiomiocitos en respuesta a cambios dinámicos de rigidez. Esta plataforma permite investigar la memoria mecánica cardíaca.

Elastómeros magnetorreológicos micromodelados para impulsar cambios en la alineación de los cardiomiocitos

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