Un ensayo microscópico de cloruro de 2,3,5-trifeniltetrazolio para un análisis preciso y fiable de la lesión miocárdica

845 vistas

11:17 min

28 de noviembre de 2025

10.3791/68596-v

28 de noviembre de 2025

845 vistas

Zheheng Ding^*¹ , Hui Zhang^*¹^,²^,³^,⁴^,⁵ , Pascal Bouvain¹ , Zhaoping Ding¹ , Ulrich Flögel¹

¹Institute of Molecular Cardiology, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University of Düsseldorf, ²Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, ³Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, ⁴Clinical Research Center for Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, ⁵Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People's Hospital, School of Medicine, Tongji University

^* These authors contributed equally

El presente ensayo de cloruro de 2,3,5-Trifeniltetrazolio (TTC) introduce un protocolo de tinción TTC refinado que permite el análisis de lesiones miocárdicas a resolución microscópica, produciendo imágenes de alta calidad que permiten una evaluación precisa y fiable del tamaño del infarto y la viabilidad del cardiomiocito a nivel celular.