Name: Un modelo de lesión por hipoxia-reoxigenación en cardioides humanos autoensamblantes (Video) | JoVE Journal
Uploaded: 2026-03-17T12:35:00.000+00:00
Duration: 10 min 41 s
Description: Aprende a generar organoides cardíacos humanos autoensamblantes y modelar lesión por hipoxia-reoxigenación usando células iPSC. Este protocolo permite estudiar respuestas a daño cardíaco y evaluar terapias regenerativas en contexto humano.

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</ol>

<table><tbody><tr><td>Solución de accutasa</td><td>Sigma-Aldrich</td><td>A6964-500ML</td><td></td></tr><tr><td>Anticuerpo anticardíaco Troponina T</td><td>Abcam</td><td>EPR20266</td><td></td></tr><tr><td>Suplemento de B-27 (50X), sin suero</td><td>Gibco</td><td>17504044</td><td></td></tr><tr><td>Suplemento de B-27, sin insulina</td><td>Gibco</td><td>A1895602</td><td></td></tr><tr><td>Lector de placas BioTek Synergy Neo2</td><td>BioTek</td><td></td><td></td></tr><tr><td>Cal-520, AM</td><td>AAT Bioquest</td><td>21131</td><td></td></tr><tr><td>CardioCount V1</td><td>Karra Lab</td><td>PMID: 38984052</td><td></td></tr><tr><td>Línea celular (Homo sapiens, masculino) - Línea iPSC DU11</td><td>Núcleo de células madre de la Universidad de Duke</td><td></td><td></td></tr><tr><td>Portaobjetos de microscopio cargados, vidrio blanco</td><td>VWR</td><td>89500-496</td><td></td></tr><tr><td>CHIR 99021</td><td>Tocris</td><td>4423</td><td></td></tr><tr><td>CloneR</td><td>Tecnologías STEMCELL</td><td>5888</td><td></td></tr><tr><td>Microplacas de Corning 96-Well Clear Ultra Low Attachment</td><td>Fisher</td><td>7007</td><td></td></tr><tr><td>Depósitos desechables de pipetos</td><td>VWR</td><td>89094-678</td><td></td></tr><tr><td>DMEM/F-12, HEPES</td><td>Gibco</td><td>11330057</td><td></td></tr><tr><td>Microscopio Fluorescente EVOS</td><td>EVOS</td><td></td><td></td></tr><tr><td>Matriz basal basal de Geltrex con factor de crecimiento reducido, libre de LDEV, cualificada para células madre</td><td>Gibco</td><td>A1413302</td><td></td></tr><tr><td>Activina humana, una proteína recombinante</td><td>PEPROTECH INC/Life Technologies</td><td>120-14E-50UG</td><td></td></tr><tr><td>IWP-2</td><td>Tecnologías STEMCELL</td><td>72124</td><td></td></tr><tr><td>Ácido L-Ascórbico (Cristalino/ACS certificado)</td><td>Fisher Scientific</td><td>A61-100</td><td></td></tr><tr><td>Anti-Vimentina para ratones</td><td>DSHB</td><td>AMF-17b</td><td></td></tr><tr><td>mTeSR Plus</td><td>Tecnologías STEMCELL</td><td>100-0276</td><td></td></tr><tr><td>Placas de cultivo celular multipozo y ndash; 12 pozo</td><td>VWR</td><td>10062-894</td><td></td></tr><tr><td>Pluronic F-127</td><td>Sigma-Aldrich</td><td>P2443</td><td></td></tr><tr><td>ReLeSR</td><td>Tecnologías STEMCELL</td><td>100-0483</td><td></td></tr><tr><td>RPMI 1640 Medio</td><td>Gibco</td><td>11875119</td><td></td></tr><tr><td>RPMI 1640 Media, sin rojo fenólico</td><td>Gibco</td><td>11835-030</td><td></td></tr><tr><td>Tiazovivina</td><td>Tecnologías STEMCELL</td><td>72254</td><td></td></tr><tr><td>Compuesto Tissue-Tek O.C.T.</td><td>SAKURA FINETEK USA INC (VWR)</td><td>25608-930</td><td></td></tr><tr><td>Microscopio Confocal Zeiss Spinning Disk</td><td>Zeiss</td><td></td><td></td></tr></tbody></table>

un modelo de lesión por hipoxia-reoxigenación en cardioides humanos autoensamblantes

A Hypoxia-Reoxygenation Injury Model in Self-Assembling Human Cardioids

Un modelo de lesión por hipoxia-reoxigenación en cardioides humanos autoensamblantes

Without rapid revascularization, ischemic heart disease leads to irreversible loss of cardiomyocytes, scar formation, and decreased cardiac function. The ...

a-hypoxia-reoxygenation-injury-model-self-assembling-human

Research

Investigación

JoVE Journal

Medicine

Medicina

Este manuscrito describe la creación de tejidos cardíacos de ingeniería definidos utilizando la expresión de marcadores de superficie y la clasificación de células. Los tejidos definidos se pueden utilizar en un biorreactor multitejido para investigar los mecanismos de la terapia celular cardíaca con el fin de proporcionar un sistema modelo funcional, pero controlado, del corazón humano.

Construcción de Engineered Definido Humanos cardíacos tejidos para estudiar los mecanismos de la terapia celular cardiaca

Aquí, presentamos un protocolo para describir un modelo de recuperación simple puente cardiopulmonar sin transfusión o agentes inotrópicos en una rata. Este modelo permite el estudio de largo plazo múltiples secuelas órgano de puente cardiopulmonar.

Un modelo de derivación cardiopulmonar de recuperación sin transfusión o agentes inotrópicos en ratas

Presentamos un modelo de cardiopatía isquémica utilizando cardiomiocitos derivados de células madre pluripotentes inducidas por humanos, junto con un método para la evaluación cuantitativa del daño tisular causado por la isquemia. Este modelo puede proporcionar una plataforma útil para la detección de fármacos y la investigación adicional sobre enfermedades cardíacas isquémicas.

Modelo de cardiopatía isquémica y comparación basada en vídeo de la contracción de cardiomiocitos utilizando cardiomiocitos derivados de hiPSC

Los distintos efectos de los diferentes grados de hipotermia en la protección miocárdica no han sido evaluados a fondo. El objetivo del presente estudio era cuantificar los niveles de muerte celular tras diferentes tratamientos de hipotermia en un modelo humano basado en cardiomiocitos, sentando las bases para futuras investigaciones moleculares en profundidad.

Evaluación in vitro de la protección miocárdica después del preaccondicionamiento de hipotermia en un modelo de miocitos cardíacos humanos

El objetivo de este protocolo es explicar y demostrar el desarrollo de un modelo microfluídico tridimensional (3D) de tejido cardíaco humano altamente alineado, compuesto por cardiomiocitos derivados de células madre co-cultivados con fibroblastos cardíacos (CF) dentro de un hidrogel biomimético a base de colágeno, para aplicaciones en ingeniería de tejido cardíaco, detección de fármacos y modelado de enfermedades.

Desarrollo de tejido cardíaco humano organizado en 3D dentro de una plataforma microfluídica

La distancia espacial es un parámetro clave en la evaluación de la lesión por hipoxia/reoxigenación en un modelo de cocultivo de capas separadas de células endoteliales y cardiomiocitarias, lo que sugiere, por primera vez, que la optimización del entorno espacial de cocultivo es necesaria para proporcionar un modelo in vitro favorable para probar el papel de las células endoteliales en la protección de los cardiomiocitos.

Desarrollo de un modelo de cocultivo celular para imitar la isquemia/reperfusión cardíaca in vitro

Aquí, describimos un protocolo para crear organoides cardíacos humanos (hHO) relevantes para el desarrollo de manera eficiente utilizando células madre pluripotentes humanas por autoorganización. El protocolo se basa en la activación secuencial de señales de desarrollo y produce tejidos cardíacos humanos altamente complejos y funcionalmente relevantes.

Generación de organoides del corazón humano autoensamblables derivados de células madre pluripotentes

Los tejidos cardíacos tridimensionales biodiseñados con cardiomiocitos derivados de células madre han surgido como modelos prometedores para estudiar el miocardio humano sano y enfermo in vitro , al tiempo que recapitulan aspectos clave del nicho cardíaco nativo. Este manuscrito describe un protocolo para la fabricación y el análisis de tejidos cardíacos de ingeniería de alto contenido generados a partir de cardiomiocitos derivados de células madre pluripotentes inducidas humanas.

Diseño de un biorreactor para mejorar la adquisición de datos y el rendimiento del modelo de tejidos cardíacos diseñados

Aquí describimos un protocolo in vitro para modelar el daño por hipoxia/reoxigenación en las células epiteliales tubulares proximales, altamente metabólicas. Este modelo está diseñado para inducir daños inducidos por estrés metabólico, que pueden medirse mediante la expresión de marcadores de daño proximal de los túbulos y la evaluación de la función respiratoria mitocondrial.

Modelado de la hipoxia/lesión por reoxigenación en células epiteliales tubulares proximales

Se describe un modelo de contusión de la lesión medular grave. Medidas pre-operatorio, operatorio y post-operatorio detalladas se describen para obtener un modelo coherente.

Un modelo Contusión de la lesión de la médula espinal severa en ratas

Aprende a generar organoides cardíacos humanos autoensamblantes y modelar lesión por hipoxia-reoxigenación usando células iPSC. Este protocolo permite estudiar respuestas a daño cardíaco y evaluar terapias regenerativas en contexto humano.

Un modelo de lesión por hipoxia-reoxigenación en cardioides humanos autoensamblantes

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