Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants

Hilda E. Hernández-Delgado; Jesús Alejandro Zamora-Briseño; Luis Joel Figueroa-Yáñez; Rafael Urrea-López

doi:10.3791/66024

In Planta Transient Transformation of Cacao Plants Using Agrobacterium tumefaciens

November 17th, 2023

Author Spotlight: Tissue Culture-Free <em>Agrobacterium</em>-Mediated Transformation in Large Woody Plants

Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants

01:53 min

•

Scale Up Culture of Transformed <i>Agrobacterium tumefaciens </i> and Agroinfiltration of Large-Sized Woody Plants

Scale Up Culture of Transformed Agrobacterium tumefaciens and Agroinfiltration of Large-Sized Woody Plants

04:02 min

•

Hilda E. Hernández-Delgado¹, Jesús Alejandro Zamora-Briseño², Luis Joel Figueroa-Yáñez³, Rafael Urrea-López¹

¹Unidad de Biotecnología Vegetal, Centro de Investigación Y Asistencia en Tecnología Y Diseño del Estado de Jalisco (CIATEJ), ²Red de Estudios Moleculares Avanzados, Instituto de Ecología, ³Unidad de Biotecnología Industrial, Centro de Investigación Y Asistencia en Tecnología Y Diseño del Estado de Jalisco (CIATEJ)

Summary
Automatic Translation

العربية (Arabic)
中文 (Chinese)
dansk (Danish)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
हिंदी (Hindi)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
norsk (Norwegian)
português (Portugese)
русский (Russian)
español (Spanish)
svenska (Swedish)
Türkçe (Turkish)

Here, we present the first protocol for localized vacuum infiltration for in vivo studies of the genetic transformation of large-sized plants. Using this methodology, we achieved for the first time the Agrobacterium-mediated in planta transient transformation of cacao.

Article

In Planta Transient Transformation of Cacao Plants Using Agrobacterium tumefaciens

Get cutting-edge science videos from JoVE sent straight to your inbox every month.