Ludwig-Maximilians University Munich (LMU) 6 articles published in JoVE Cancer Research Strategy for Biobanking of Ovarian Cancer Organoids: Addressing the Interpatient Heterogeneity Across Histological Subtypes and Disease Stages Fabian Trillsch*1,2, Juliane Reichenbach*1, Bastian Czogalla1, Fabian Kraus1, Alexander Burges1, Sven Mahner1,2, Mirjana Kessler1,2 1Department of Obstetrics and Gynecology, University Hospital, Ludwig-Maximilian-University (LMU) Munich, 2German Cancer Consortium (DKTK), Partner site Munich (LMU), a partnership between the German Cancer Research Center (DKFZ) and the University Hospital of LMU Munich This protocol offers a systematic framework for the establishment of ovarian cancer organoids from different disease stages and addresses the challenges of patient-specific variability to increase yield and enable robust long-term expansion for subsequent applications. It includes detailed steps for tissue processing, seeding, adjusting media requirements, and immunofluorescence staining. Medicine Microdissection and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins Hannes E. Villgrater*1,2,3, Ruibing Xia*1,2,3, Aparna Sharma Chivukula1,2,3, Philipp Tomsits*1,2,3, Sebastian Clauss*1,2,3,4 1University Hospital Munich, Department of Medicine I, Ludwig-Maximilians-University Munich (LMU), 2Walter Brendel Center of Experimental Medicine (WBex), LMU Munich, 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 4Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU), LMU Munich This protocol demonstrates microscopy-guided isolation and immunofluorescence staining of murine pulmonary veins. We prepare tissue samples containing the left atrium, pulmonary veins, and the corresponding lungs and stain them for cardiac Troponin T and Connexin 43. Biochemistry An Improved Method to Isolate Mitochondrial Contact Sites Siavash Khosravi*1,2, Johanna Frickel*1, Max E. Harner1 1Department of Cell Biology, Biomedical Center, Medical Faculty, Ludwig-Maximilians University Munich, 2Cardio-Metabolic Diseases, Boehringer Ingelheim Pharma GmbH & Co. KG Mitochondrial contact sites are protein complexes that interact with mitochondrial inner and outer membrane proteins. These sites are essential for the communication between the mitochondrial membranes and, thus, between the cytosol and the mitochondrial matrix. Here, we describe a method to identify candidates qualifying for this specific class of proteins. Editorial Using C. elegans to Monitor Proteostasis Imbalances Cindy Voisine1, Carmen Nussbaum-Krammer2 1Department of Biology, Northeastern Illinois University, 2Department of Anatomy II, Faculty of Medicine, Ludwig-Maximilians-University of Munich Immunology and Infection Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles Qin Xiao*1, Yuheng Liu*1, Dandan Zhang1, Chao Li1, Qihua Yang1, Dongshui Lu1, Weijun Zhang1, Joseph Rosenecker2, Quanming Zou1, Yang Li3, Shan Guan1 1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University A self-assembled peptide-poloxamine nanoparticle (PP-sNp) is developed using a microfluidic mixing device to encapsulate and deliver in vitro transcribed messenger RNA. The described mRNA/PP-sNp could efficiently transfect cultured cells in vitro. Medicine Real-Time Electrocardiogram Monitoring During Treadmill Training in Mice Philipp Tomsits*1,2,4, Aparna Sharma Chivukula*1,2, Kavi Raj Chataut*1,2, Agus Simahendra3, Ludwig T. Weckbach1,2,3, Stefan Brunner1, Sebastian Clauss1,2,4 1Department of Medicine I, University Hospital Munich, Ludwig-Maximilians University Munich (LMU), 2DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), 3Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-University, 4Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians University Munich (LMU) Electrocardiogram (ECG) is the key variable to understanding cardiac electrophysiology. Physical exercise has beneficial effects but may also be harmful in the context of cardiovascular diseases. This manuscript provides a method of recording real-time ECG during exercise, which can serve to investigate its effects on cardiac electrophysiology in mice.
