University of Campania Luigi Vanvitelli 1 article published in JoVE Developmental Biology HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis Maria Teresa Gentile1, Olga Pastorino1, Maurizio Bifulco2, Luca Colucci-D'Amato1 1Laboratory of Cellular and Molecular Neuropathology, Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 2Department of Molecular Medicine and Biotechnologies, University of Naples "Federico II" Naples Here, we evaluate the effects of the water extract of Ruta graveolens on vessel network formation by using a tube formation assay on a gelled basement matrix.