Creighton University School of Dentistry 1 article published in JoVE Genetics Inducible and Reversible Dominant-negative (DN) Protein Inhibition Shikha Tarang1, Umesh Pyakurel1, Songila M.S.R. Doi1, Michael D. Weston1, Sonia M. Rocha-Sanchez1 1Department of Oral Biology, Creighton University School of Dentistry Here we present a protocol to develop a dominant-negative inducible system, in which any protein can be conditionally inactivated by reversibly overexpressing a dominant-negative mutant version of it.