Temple University School of Medicine 1 article published in JoVE Biology An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation Mingxiao Feng1, Sara Divall3, Sheng Wu1,2 1Department of Pediatrics, Johns Hopkins University School of Medicine, 2Department of Physiology, Temple University School of Medicine, 3 Primary hepatocytes are a valuable tool to study liver response and metabolism in vitro. Utilizing commercially available reagents, an improved time- and labor-efficient protocol for mouse primary hepatocyte isolation was developed.