Alcon Vision, LLC 1 article published in JoVE Medicine Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation Rekha Rangarajan1, Howard A. Ketelson1, Richard Do2, David J. McCanna2, Adeline Suko2, Daryl Enstone2, Lakshman N. Subbaraman1, Jaya Dantam2, Lyndon W. Jones2 1Alcon Vision, LLC, 2Centre for Ocular Research and Education (CORE), School of Optometry & Vision Science, University of Waterloo The purpose of this protocol is to evaluate if different artificial tear formulations can protect human corneal epithelial cells from desiccation using an in vitro model. After exposure to artificial tear formulations and desiccation, human corneal epithelial cells are assessed for metabolic activity.