Name: how the mitotic spindle separates chromosomes
Uploaded: 2021-04-07T12:12:01.000Z
Duration: 2 min 27 s
Channel: JoVE

Le fuseau mitotique sépare les chromatides sœurs et les déplace vers les côtés opposés de la cellule pendant l’anaphase de la mitose.
Les structures fondamentales du fuseau mitotique sont des cylindres creux appelés microtubules. Deux ensembles de microtubules sont disposés aux extrémités opposées, ou pôles, du fuseau mitotique.
Chaque microtubule a une extrémité négative et une extrémité positive. Les extrémités négatives des microtubules se rejoignent au centre des pôles du fuseau. Les extrémités positives s’étendent vers l’extérieur à partir des pôles.
Il existe différents types de microtubules avec des positions et des rôles distincts dans le fuseau mitose.
Les microtubules kinétochores lient les chromosomes au pôle fusiforme en se fixant à leurs extrémités positives aux kinétochores. Les kinétochores sont de grands complexes protéiques assemblés au niveau du centromère chromatidide, une séquence d’ADN spécialisée qui relie les chromatides sœurs.
La disposition des microtubules interpolaires ressemble à une paire de mains jointes. L’extrémité positive d’un microtubule interpolaire chevauche l’extrémité positive d’un autre microtubule interpolaire s’étendant à partir du pôle opposé. Les protéines motrices s’associent aux microtubules interpolaires pour diriger l’assemblage du fuseau.
Les microtubules astraux ancrent le fuseau dans la cellule. Ces microtubules ressemblent collectivement à une étoile : chaque extrémité positive se projetant vers l’extérieur du pôle du fuseau vers le cortex cellulaire.
Dans la plupart des cellules animales, les microtubules sont organisés autour d’un organite appelé centrosome. Un centrosome est présent à chaque pôle de broche.
Un centrosome est constitué de deux centrioles entourés d’une masse informe de protéines appelée matrice péricentriolaire. Le centrosome produit, organise et ancre les microtubules dans la cellule.
Deux familles de protéines motrices font partie intégrante de la construction et du fonctionnement du fuseau mitotique : les protéines liées à la kinésine et la dynéine. En règle générale, les protéines liées à la kinésine se déplacent vers les extrémités positives des microtubules et la dynéine se déplace vers les extrémités négatives.
La polarité intrinsèque du fuseau mitotique et de ses microtubules facilite la ségrégation mitotique des chromosomes, préparant la cellule à la division.

<ol>
	<li>Heald, Rebecca et Alexey Khodjakov. 2015. « Trente ans de recherche et de capture : la simplicité complexe de l’assemblage du fuseau mitotique. » Le Journal de biologie cellulaire 211 (6) : 1103-11. <a href="https://doi.org/10.1083/jcb.201510015">https://doi.org/10.1083/jcb.201510015</a>.</li>
	<li>Petry, Sabine. 2016. « Mécanismes de l’assemblage du fuseau mitotique. » Revue annuelle de biochimie 85 (1) : 659-83. <a href="https://doi.org/10.1146/annurev-biochem-060815-014528">https://doi.org/10.1146/annurev-biochem-060815-014528</a>.</li>
</ol>

le fuseau mitotique

The Mitotic Spindle

Le fuseau mitotique – ou appareil à fuseau – est une structure cytosquelettique eucaryote composée de longues fibres protéiques appelées microtubules. Formé lors de la division cellulaire, le fuseau sépare les chromatides sœurs et les déplace vers les extrémités opposées d'une cellule parentale, où les chromosomes désormais individuels sont distribués vers deux noyaux de cellules filles.

La configuration bipolaire du fuseau mitotique facilite la ségrégation chromosomique, préparant la cellule à la division. Un mécanisme qui assure la formation du fuseau mitotique bipolaire repose sur les centrosomes.

Certaines cellules, comme les ovocytes de vertébrés et les cellules végétales supérieures, sont dépourvues de centrosomes ; cependant, la plupart des cellules animales possèdent deux centrosomes lorsqu’elles entrent en mitose. Chaque centrosome s'associe à un arrangement circulaire de microtubules aux extrémités opposées —ou pôles— du fuseau mitotique. En d’autres termes, les centrosomes nucléent les microtubules.

Les protéines motrices— notamment les kinésines et la dynéine— opèrent généralement au niveau ou à proximité des extrémités des microtubules et facilitent la formation du fuseau mitotique bipolaire et la séparation des chromatides sœurs.

Par exemple, les moteurs kinésine-5 situés au niveau de la zone médiane du fuseau s'attachent à et séparent deux microtubules s'étendant à partir des pôles opposés du fuseau ; ce processus favorise la bipolarité et l'allongement du fuseau en éloignant les pôles du fuseau les uns des autres.

On pense que l’activité de la kinésine-5 est contrebalancée par la kinésine-14. Les moteurs Kinesin-14 tirent sur les microtubules s’étendant des pôles opposés, rapprochant ainsi efficacement les pôles. L'activité coordonnée de ces moteurs permet à la broche de s'assembler correctement.

La kinésine-4 et la kinésine-10 sont des chromokinésines, des kinésines qui peuvent s'associer aux chromosomes mitotiques. La kinésine-4 et la kinésine-10 s'associent aux bras chromosomiques, écartant les chromosomes et le pôle du fuseau.

Les dynéines organisent les microtubules dans diverses parties de la cellule. Par exemple, ils relient les microtubules astraux au cytosquelette d’actine, éloignant les pôles du fuseau les uns des autres.

La structure, l'organisation et les composants du fuseau mitotique permettent aux chromatides sœurs de se séparer, préparant ainsi la cellule à une division appropriée.

Le fuseau mitotique

Le fuseau mitotique est une structure cytosquelettique eucaryote composée de longues fibres protéiques appelées microtubules. Formé...

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Biologie moléculaire

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Cell Division

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how the mitotic spindle separates chromosomes

The mitotic spindle is a microtubule-based structure that helps separate chromosomes during cell division. It forms in eukaryotic cells and moves sister chromatids to opposite ends of the parent cell. This leaves each new cell nucleus with the correct genetic material.

A bipolar spindle has two poles, and that shape is important for chromosome segregation. In many animal cells, two centrosomes are present when mitosis begins. Each centrosome helps organize a circular set of microtubules at one pole, which means centrosomes nucleate microtubules. Some cells, including vertebrate oocytes and higher plant cells, do not have centrosomes.

Motor proteins help build and position the spindle. Kinesins and dynein work at or near microtubule ends and support bipolar spindle formation as well as sister chromatid separation. Kinesin-5 motors at the spindle midzone slide two microtubules apart, which pushes the poles away from each other and helps the spindle elongate.

Kinesin-14 acts in the opposite way. It pulls on microtubules that extend from opposite poles and brings the poles closer together. This balance between kinesin-5 and kinesin-14 helps the spindle assemble correctly. Kinesin-4 and kinesin-10 are chromokinesins, which means they can bind to mitotic chromosomes. They attach to chromosome arms and help push the chromosomes and spindle poles apart.

Dyneins also take part in spindle organization. They link astral microtubules to the actin cytoskeleton and help move the spindle poles away from each other. Together, the spindle structure, its poles, and its motor proteins allow sister chromatids to separate and prepare the cell for proper division.

How the Mitotic Spindle Separates Chromosomes

how-the-mitotic-spindle-separates-chromosomes

mitosis and cytokinesis

Mitosis and Cytokinesis Stages

Mitosis and cytokinesis are the two main steps of cell division. Mitosis is the process that divides the nucleus, while cytokinesis splits the rest of the cell. During mitosis, the cell’s genetic material is separated into two sets. This helps form two new nuclei with the same genetic information. Cytokinesis then completes division by separating the cytoplasm, which creates two daughter cells. These stages work together to make cell division possible in growing and repairing tissues.

duplication of chromatin structure

Chromatin Rebuilding During DNA Replication

Chromatin rebuilding during DNA replication helps cells copy DNA and pass on chromosome structure to daughter cells. During cell division, the genome must be disrupted and then reassembled across the whole chromosome. This process allows chromatin to be inherited, rebuilt, and maintained in new cells. The basic unit of chromatin is the nucleosome. A nucleosome contains DNA wrapped around an octamer of histone proteins, with short linker DNA between neighboring nucleosomes. The histone proteins...

cohesins

Cohesins in Meiosis and Genetic Disorders

Cohesin protein complexes act like molecular glue that keeps sister chromatids together. They are important in both mitosis and meiosis. In mitosis, cohesin complexes are removed from chromosomes before anaphase begins. Meiosis has two rounds of chromosome separation. Meiosis I separates homologous chromosomes, while Meiosis II separates sister chromatids. This process produces four daughter cells. The Meiosis I cohesin complex is made of four subunits: Smc1, Smc3, Rec8, and Scc3. Rec8...

condensins

How Condensins Shape Mitotic Chromosomes

Condensins shape chromosomes during mitosis, the stage when a cell divides. These large protein complexes use ATP, the cell’s energy molecule, to help turn loose post-interphase DNA into compact, separate chromosomes. They organize and segregate chromosomal DNA so it can be passed on correctly. Plant and animal cells contain two condensin complexes: condensin I and condensin II. Each complex has five subunits. Two are SMC, or Structural Maintenance of Chromosomes, subunits. One is a kleisin...

the mitotic spindle

The mitotic spindle is a microtubule-based structure that helps separate chromosomes during cell division. It forms in eukaryotic cells and moves sister chromatids to opposite ends of the parent cell. This leaves each new cell nucleus with the correct genetic material. A bipolar spindle has two poles, and that shape is important for chromosome segregation. In many animal cells, two centrosomes are present when mitosis begins. Each centrosome helps organize a circular set of microtubules at one...

centrosome duplication

Centrosome Duplication in the Cell Cycle

Centrosome duplication is a key part of the animal cell cycle. The centrosome is the main microtubule organizing center, or MTOC, in animal cells. It contains two cylindrical centrioles at its core. Each centriole has nine sets of three microtubules held together by proteins. The two centrioles sit at right angles and are surrounded by the pericentriolar matrix, or PCM, a shapeless protein cloud. Duplication starts early in the cell cycle so each daughter cell can receive a centrosome after...

microtubule instability

Microtubule Growth and Shrinkage

Microtubules are hollow, cylindrical filaments in the cell. They are about 25 nm wide and can range from 200 nm to 25 μm in length. Their main building blocks are GTP-bound tubulin subunits that form αβ-heterodimers, which then assemble into protofilaments. These protofilaments join by lateral bonds to create stable microtubule cylinders. Microtubules are highly dynamic because they repeatedly assemble and break down. They can even grow on one end while shrinking on the other at the same time.

spindle assembly

How the Mitotic Spindle Forms

The mitotic spindle forms through centrosome-mediated, chromatin-mediated, and microtubule-mediated pathways. These pathways often work at the same time. Together, they build a strong spindle apparatus for cell division. In most cells, centrosomes are the main sites where microtubules begin to grow. During the G2 to prophase transition, centrosomes mature and make more microtubules. The growing microtubules extend from both centrosomes, and their plus-ends search for chromosomes by attaching...

attachment of sister chromatids

Kinetochore-Microtubule Binding in Mitosis

Kinetochore-microtubule binding helps chromosomes line up and separate during mitosis. After the nuclear envelope breaks down, condensed chromosomes are exposed to the bipolar microtubules of the mitotic spindle. The kinetochore is a large, disc-shaped protein complex at the centromere region of each sister chromatid, and it serves as the microtubule binding site. Usually, the plus-end of a single microtubule inserts into the kinetochore. In some cases, a kinetochore first makes lateral...

forces acting on chromosomes

Chromosome Movement During Mitosis

Chromosome movement during mitosis depends on several small forces that act at different times and positions in the cell. In prometaphase, these forces help chromosomes move to the equatorial plane and line up at the metaphase plate. Because the forces change with location and stage, chromosome behavior also changes as mitosis continues. Microtubules and motor proteins create two main kinds of force on chromosomes: poleward force and anti-poleward force, also called polar-ejection force.

separation of sister chromatids

Anaphase Start and Chromatid Release

Anaphase starts when sister chromatids are released from one another and pulled toward opposite spindle poles. Before that happens, cohesion along the chromosomal arms is reduced from prophase to metaphase, which helps the sister chromatids resolve. A residual cohesin link still holds them together until the metaphase-to-anaphase transition. This leftover connection helps prevent early separation and reduces the risk of aneuploidy, or the wrong number of chromosomes, in daughter cells. At the...

the spindle assembly checkpoint

How Cells Safeguard Chromosome Separation

The spindle assembly checkpoint helps cells safeguard chromosome separation during anaphase. It acts as a molecular surveillance system. The checkpoint checks whether the steps needed for chromosome segregation are complete before the cell moves on. Many proteins work together to control this checkpoint. If mutations affect these proteins, a cell may enter anaphase too early. Chromosomes can then separate before the needed steps are finished. When this happens, the daughter cells may receive...

anaphase a and b

Chromatid Movement by Microtubule Forces

Chromatid movement during anaphase depends on microtubule forces at the spindle. Kinetochore microtubules grow from the spindle poles, and their plus ends attach to kinetochores on sister chromatids. The kinetochore protein complex Ndc80 makes low-affinity links with the microtubule plus end.

the contractile ring

How Cells Build the Contractile Ring

Contractile rings are built from microfilaments and help separate daughter cells during cytokinesis. This ring forms as the cell cycle moves forward, but the exact timing and coordination with other cell cycle events are still not fully understood. RhoA, a small GTPase in the Ras superfamily, controls contractile ring assembly and function. The inactive form of RhoA is bound to GDP. A guanine nucleotide exchange factor, or Rho-GEF, is located in the cortex region, where the next cell division...

determining the plane of cell division

How Cells Set the Division Plane

How cells set the division plane during cell division affects daughter cell size and, in many cases, cell fate. The plane of division is perpendicular to the plane of chromosome segregation. Different organisms use different cues to place it, based on their shape and needs. In animal cells, the cleavage furrow forms along the division plane from the cell cortex, the area just below the plasma membrane. The mitotic spindle is the main guide for this position. If the spindle axis changes, the...

the phragmoplast

How Plant Cells Build a New Wall

Plant cells build a new wall during cytokinesis with help from the phragmoplast. This plant structure forms from the central spindle and related cell parts as the cell finishes dividing. In animal cells, the comparable structure is the midbody, but in plants the phragmoplast expands outward to guide new wall formation. The mature phragmoplast has a donut-like shape. It has an outer leading region, a middle transition zone, and an inner lagging region. New microtubules are assembled...

distribution of cytoplasmic content

How Organelles Are Split During Cytokinesis

Cytokinesis splits a cell’s chromosomes and organelles into two daughter cells. Before cell division, organelles grow and divide. They cannot be made from scratch, so each daughter cell must inherit at least one copy to survive. Many details of this organelle sorting process are still not fully known. The cytoplasm contains organelles, salts, proteins, and water. Small organelles such as peroxisomes and lysosomes can be shared by simple diffusion. Because cells contain many copies of these...

anaphase promoting complex

APC Activates Chromosome Separation

The anaphase-promoting complex, or APC, helps control cell cycle progression by destroying specific proteins at the right time. These proteins are tagged with ubiquitin by ubiquitin ligases and then broken down by the proteasome. The SCF (Skp1/Cullin/F-box) complex and APC are two major ubiquitin ligases involved in this process. SCF stays active throughout the cell cycle, but APC turns on during the metaphase-to-anaphase transition. APC becomes active when Cdc20 or Cdh1 binds to it. These...

key terms and concepts

Learn how the mitotic spindle uses microtubules, centrosomes, kinesins, and dynein to separate sister chromatids during cell division.

How the Mitotic Spindle Separates Chromosomes | JoVE K12

molecular biology

JoVE Core Biologie Moléculaire illustre les processus moléculaires au sein de la cellule grâce à des leçons vidéos claires et concises. Les applications de ces concepts sont ensuite démontrées grâce à des expériences concrètes en laboratoire.

How the Mitotic Spindle Separates Chromosomes

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