Protocol
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
Valeria Purpura1, Elena Bondioli1, Antonio Graziano2, Letizia Trovato2, Davide Melandri1, Martina Ghetti1, Andrea Marchesini3, Maria Gabriella Cusella De Angelis4,5, Laura Benedetti4,5, Gabriele Ceccarelli4,5, Michele Riccio3
1Burns Centre and Emilia Romagna Regional Skin Bank, 2Human Brain Wave srl, 3Plastic and Reconstructive Surgery, AOU “Ospedali Riuniti”, 4Department of Public Health, Experimental Medicine, Anatomy and Forensic, University of Pavia, 5C.H.T Centre for Health Technologies, University of Pavia
The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.
Air-sampled Filter Analysis for Endotoxins and DNA Content
Naama Lang-Yona1,2, Yinon Mazar1, Michal Pardo1, Yinon Rudich1
1Department of Earth and Planetary Sciences, Weizmann Institute of Science, 2Multiphase Chemistry Department, Max Planck Institute
Two complementary analyses of atmospheric biological particles from air sampled filters are described herein: the extraction and detection of endotoxin, and of DNA.
RNAi Trigger Delivery into Anopheles gambiae Pupae
Kimberly Regna1, Rachel M. Harrison1, Shannon A. Heyse1, Thomas C. Chiles1, Kristin Michel2, Marc A. T. Muskavitch1,3
1Biology Department, Boston College, 2Division of Biology, Kansas State University, 3Discovery Research, Biogen
RNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during pupal development.
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
Ashley Ulm1, Christopher N. Mayhew2, Jason Debley3, Gurjit K. Khurana Hershey4, Hong Ji1,4
1Pyrosequencing Core, Cincinnati Children's Hospital, 2Division of Developmental Biology, Cincinnati Children's Hospital, 3Division of Pulmonary Medicine, Seattle Children's Hospital, 4Division of Asthma Research, Cincinnati Children's Hospital
This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).
Disclosures
No conflicts of interest declared.