Multiwell Platform

A multiwell platform is a laboratory format that organizes multiple small-volume reactions, cultures, or samples in separate wells, enabling parallel experiments with consistent handling and measurement. Each well acts as an independent microenvironment, while standardized geometry, controlled liquid volumes, and compatible imaging or plate-based readouts support simultaneous analysis across many conditions. In bioengineering, multiwell platforms are used to culture cells, evaluate biomaterials, optimize tissue-engineering conditions, and screen drugs or biological factors. Their scalable design reduces reagent use, improves experimental throughput, and enables systematic comparisons of variables such as composition, concentration, and mechanical or biochemical cues.

Multiwell Platform - Related Videos

Research

JoVE Journal - Biology

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format

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Cited by 3 •

2016

Here, sporulation of Saccharomyces cerevisiae is carried out in a 96 multiwell format.

A Multiwell Liquid-Based Assay for Screening C. elegans Knockdowns

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2025

Source: Anderson, Q. L. et al. A High-throughput, High-content, Liquid-based C. elegans Pathosystem. J. Vis. Exp. (2018)This video demonstrates a high-throughput screening method using Caenorhabditis elegans to study gene knockdowns during host-pathogen interactions. It outlines the procedure for preparing knockdown worms and transferring them to assay plates to evaluate the effects of pathogen-induced worm killing.

Research

JoVE Journal - Bioengineering
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Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

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Cited by 5 •

2019

This article contains a set of protocols for the development of human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) networks cultured on multiwell MEA plates to reversibly electroporate the cell membrane for action potential measurements. High-throughput recordings are obtained from the same cell sites repeatedly over days.

The Water Maze with Platform Relocation: A Method to Assess Working Memory

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2023

In this video, we describe the Morris water maze test with platform relocation, a navigational task used to study working memory in rodents.

Simple Polyacrylamide-based Multiwell Stiffness Assay for the Study of Stiffness-dependent Cell Responses

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Cited by 56 •

2015

Here, a method that enables quick, efficient, and inexpensive preparation of polyacrylamide gels in a multiwell plate format is described. The method does not require any specialized equipment and could be easily adopted by any research laboratory. It would be particularly useful in research focused on understanding stiffness-dependent cell responses.

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