Neonatal Mouse Spleens
Neonatal Mouse Spleens - Related Videos
04:47
A Mouse Model of Vascularized Heterotopic Spleen Transplantation
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2025
The video demonstrates a surgical method for generating a mouse model of vascularized heterotopic spleen transplantation. The abdominal cavity of an anesthetized donor mouse is surgically opened to remove the spleen. The inferior vena cava and infrarenal aorta are identified, their lumbar branches are ligated, and openings are made for the heterotopic transplantation of a spleen from a congenic donor mouse. Upon completing vascular anastomosis and restoring blood flow to the implanted spleen,...
02:03
Assessing Bacterial Burden in the Mouse Spleen for Infection Studies
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2026
Source: Madenspacher, J. H. and Fessler, M. B. A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice. J. Vis. Exp. (2016)This video demonstrates the process of quantifying bacterial load in the spleen of an infected mouse to assess the severity of infection. The homogenized spleen is serially diluted and plated to determine total bacterial burden. The resulting data provide insights into host-pathogen interactions within...
05:18
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
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Cited by 22 •
2008
This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.
07:04
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Cited by 19 •
2017
We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
07:50
Isolation and Culture of Neonatal Mouse Cardiomyocytes
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Cited by 187 •
2013
Primary mouse cardiomyocyte cultures are one of the pivotal tools for the investigation of myofibrillar organization and function. The following protocol describes the isolation and culture of primary cardiomyocytes from neonatal mouse hearts. The resulting cardiomyocyte cultures may be subsequently used for a variety of biomechanical, biochemical and cell-biological assays.
FAQs
The key developmental transition is a shift in hematopoietic activity after birth. As this activity changes, the spleen does not remain static: lymphocyte populations expand, and immune compartments become progressively organized. These linked changes provide a biological framework for interpreting neonatal spleen data, because cellular composition and tissue organization can reflect developmental stage as well as experimental exposure.
Lymphocyte expansion and compartment organization indicate that immune maturation involves both changing cell numbers and increasing tissue structure. Examining these features together can reveal whether a developmental process affects population growth, spatial organization, or both. This distinction helps researchers interpret how early immune systems become established and how developmental signals shape the spleen after birth.
Developmental timing matters because neonatal spleens undergo rapid structural and functional changes during the early postnatal period. A finding in tissue, cell populations, or gene expression may therefore reflect normal maturation, an altered developmental trajectory, or a response to environmental exposure. Relating observations to the postnatal stage is essential for distinguishing these possibilities.
Researchers can examine the tissue itself, identify changes in cell populations, and analyze gene expression. Together, these approaches connect spleen structure with cellular composition and molecular activity. Using more than one level of analysis can provide a broader view of immune development and hematopoiesis, rather than attributing an observed change to a single biological feature.
This model allows investigators to examine how an early developing immune system responds in the context of infection or inflammation. Tissue observations, cell-population analysis, and gene-expression studies can each contribute different evidence about that response. The resulting data help place inflammatory or infectious changes within the broader process of postnatal immune maturation.
Neonatal spleen studies can show how genetic disease affects immune development, hematopoiesis, tissue organization, or gene expression during an early postnatal window. They also provide a foundation for investigating experimental interventions. Because the organ is undergoing rapid maturation, researchers can evaluate how an intervention relates to developmental immune processes and potential disease-associated changes.