Fluorescent Tumor Cells
Fluorescent Tumor Cells - Related Videos
03:19
Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro
0 Views •
2023
The following video describes a technique of transduction to label (patient-derived xenografts) PDX tumor cells, which is used for introducing lentivirus expressing green-fluorescent protein and luciferase reporters into the tumor cell in vitro.
09:04
In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles
0 Views •
Cited by 13 •
2014
We describe a multi-angle rotational optical imaging (MAROI) system for in vivo quantitation of a fluorescent marker delivered by saposin C (SapC)-dioleoylphosphatidylserine (DOPS) nanovesicles. Employing mouse models of cancer and arthritis, we demonstrate how the MAROI signal curve analysis can be used for the precise mapping and biological characterization of disease processes.
05:08
In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy
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Cited by 5 •
2013
In this paper, we present a method to analyze tumor microvessels in vivo using dynamic contrast-enhanced fluorescence videomicroscopy. Two quantitative parameters were acquired: functional capillary density reflecting the vascularity of the tumor, and index leakage reflecting the leakiness of the endothelial wall.
03:10
In Vivo Fluorescence Imaging to Localize Antibodies in a Mouse Tumor Xenograft Model
0 Views •
2025
The video demonstrates a fluorescence imaging method for in vivo antibody localization in a mouse tumor xenograft model. It involves the injection of tumor cells in a mouse to generate solid xenografts beneath the skin. Subsequent injection of fluorescent antibodies confirms specific localization through enhanced fluorescence signals within the tumor xenograft.
05:08
Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo
0 Views •
2023
This video demonstrates the protocol for implanting green fluorescent protein-expressing pancreatic cancer cells orthotopically into the pancreas of the mouse. The orthotopic mouse model helps in investigating the efficacy of novel therapeutics on primary and metastatic tumors.
FAQs
The signal arises when fluorescent proteins or chemical dyes absorb excitation light and then emit light at defined wavelengths. Imaging systems detect that emitted light, distinguishing labeled cancer cells from their surroundings. Because excitation and emission are linked, researchers can use the resulting fluorescence to determine where cells are located and observe their behavior during an experiment.
Both fluorescent proteins and chemical dyes provide detectable signals, but they represent different labeling approaches. Fluorescent proteins are associated with cells engineered to produce fluorescence, whereas dyes label cells through a chemical signal. This distinction allows researchers to select a strategy suited to tracking cancer cells, visualizing populations, or examining changes in living systems.
Fluorescence makes dynamic cancer-cell behaviors visible rather than limiting observation to a single endpoint. Researchers can follow changes in cell location and movement, examine growth, and observe interactions with surrounding tissues. These observations help connect cellular behavior with tumor development, invasion, and metastasis, providing a visual basis for studying how cancer progresses through living systems.
Preparation begins by labeling cancer cells with a fluorescent dye or engineering them to produce a fluorescent protein. The marked cells are then examined with microscopy or another imaging method capable of detecting the emitted signal. This workflow creates a visible cell population that researchers can follow in living systems and use for subsequent analysis.
They are especially useful when a study needs to monitor tumor behavior over time. Investigators can apply them to research on tumor development, local invasion, metastasis, and interactions between cancer cells and surrounding tissues. Their signals also support studies of treatment response, helping reveal how tumors change during experimental therapy evaluation.
The visible signal provides two practical advantages beyond observation. Researchers can track how fluorescent tumor populations change after exposure to an experimental therapy, and they can use the signal to identify and isolate cancer-cell populations. Together, these capabilities connect treatment effects with specific cells and make it easier to examine changes in tumor biology.