Cellular Targeting
Cellular Targeting - Related Videos
08:45
Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors
0 Views •
Cited by 4 •
2020
The ability to assess target engagement by candidate inhibitors in intact cells is crucial for drug discovery. This protocol describes a 384 well format cellular thermal shift assay that reliably detects cellular target engagement of inhibitors targeting either wild-type SHP2 or its oncogenic variants.
09:26
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
0 Views •
Cited by 6 •
2011
This protocol will demonstrate the extraction and analysis of free and esterified bioactive fatty acids from cells. Fatty acids are accurately quantified using stable isotope dilution, chiral liquid chromatography, electron capture atmospheric chemical ionization multiple reaction monitoring mass spectrometry (SID-LC-ECAPCI-MRM/MS).
11:00
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
0 Views •
Cited by 31 •
2018
This report describes a fast and reliable method for validating mRNA targets of cellular miRNAs. The method uses synthetic biotinylated Locked Nucleic Acid (LNA)-based miRNA mimics to capture target mRNA. Subsequently, streptavidin-coated magnetic beads are employed to pulldown the target mRNA for quantification by qPCR polymerase chain reaction.
05:00
Cellular Respiration - Concepts
0 Views •
2019
Autotrophs and Heterotrophs Living organisms require a continuous input of energy to maintain cellular and organismal functions such as growth, repair, movement, defense, and reproduction. Cells can only use chemical energy to fuel their functions, therefore they need to harvest energy from chemical bonds of biomolecules, such as sugars and lipids. Autotrophic organisms, namely plants, algae, and photosynthetic and chemosynthetic bacteria, convert inorganic materials into such biomolecules by...
11:58
Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting
0 Views •
Cited by 5 •
2018
Viral-derived peptides coupled to antibody-conjugates (ACs) is an approach gaining momentum due to the potential of delivering molecular payloads with increased tumor cell accumulation. Utilizing common methods to evaluate peptide conjugation, AC and payload intracellular accumulation, and tumor targeting, this protocol helps researchers during the key initial development phases.
FAQs
Selectivity depends on matching a targeting element with a distinguishing feature of the desired cell. The relevant interaction may involve a ligand binding a cell-surface receptor, an antibody recognizing an antigen, or an engineered carrier interacting with membrane components. The quality of this molecular match influences where the chemical intervention accumulates and how precisely it acts.
Recognition is not only a positioning event. Binding can trigger cellular uptake, promote release from a delivery system, or concentrate chemical activity near the selected cell. These outcomes determine whether the active molecule enters the cell, becomes available at a local site, or remains associated with the carrier, directly shaping intervention effectiveness.
Directing activity toward selected cells can concentrate the intervention where it is intended to work while limiting exposure elsewhere. This balance may improve treatment effectiveness and reduce unwanted distribution of chemical activity. In research, the same selectivity helps distinguish effects associated with particular cell populations from effects occurring more broadly.
These components provide different routes for selective recognition. Ligands interact with cell-surface receptors, antibodies recognize antigens, and engineered carriers associate with membrane components. Their choice connects molecular recognition to the intended outcome, such as uptake, release, or localized activity, making them central design elements in targeted chemical systems.
Chemists apply these principles when developing targeted drugs, imaging probes, and nanoparticle-based delivery systems. The design connects a recognition feature with a chemical payload or detectable function, allowing the resulting system to act or report more selectively. This approach supports both therapeutic development and investigation of biological processes in selected cells.
Targeted systems can reveal whether a chemical effect is associated with a particular cell type or biological site. By concentrating activity in selected cells, they can clarify biological mechanisms and support more precise disease research. When used as imaging probes, they also connect selective recognition with observation of the intended cellular location.