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Genetics

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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
 

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Article doi: 10.3791/57786
June 12th, 2018

Summary June 12th, 2018

This report describes a fast and reliable method for validating mRNA targets of cellular miRNAs. The method uses synthetic biotinylated Locked Nucleic Acid (LNA)-based miRNA mimics to capture target mRNA. Subsequently, streptavidin-coated magnetic beads are employed to pulldown the target mRNA for quantification by qPCR polymerase chain reaction.

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