Optical Delay Line Alignment
Optical Delay Line Alignment - Related Videos
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
07:05
The Precision of Visual Working Memory with Delayed Estimation
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Human memory is limited. Throughout most of its history, experimental psychology has focused on investigating the discrete, quantitative limits of memory—how many individual pieces of information a person can remember. Recently, experimental psychologists have also become interested in more qualitative limits—how precisely is information stored? The concept of memory precision can be both intuitive and elusive at once. It is...
07:47
Measuring Delay Discounting in Humans Using an Adjusting Amount Task
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Cited by 55 •
2016
Delay discounting refers to a decline in the value of a reward when it is delayed relative to when it is immediately available. We outline a computer-based delay discounting task that is easy to implement and allows for the quantification of the degree of delay discounting in human participants.
07:02
Alignment of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina
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Cited by 3 •
2023
The present protocol outlines the steps for aligning in vivo visible-light optical coherence tomography fibergraphy (vis-OCTF) images with ex vivo confocal images of the same mouse retina for the purpose of verifying the observed retinal ganglion cell axon bundle morphology in the in vivo images.
13:39
Optical Trapping of Nanoparticles
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Cited by 7 •
2013
The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.
FAQs
Mirrors and retroreflectors determine whether the beam remains collinear, meaning its segments continue along the intended common line. Their adjustment also affects how much light reaches the detector or sample. Optimizing these components therefore serves two linked purposes: preserving the geometric path and maximizing transmitted intensity, which strengthens the measured optical signal.
Changing the optical path length changes the travel distance experienced by the beam and consequently varies the delay between pulses or between excitation and measurement. This adjustable timing allows an experiment to examine a sample at different stages after photoexcitation, making the delay line relevant to measurements of time-dependent molecular behavior.
Beam collinearity keeps the optical path directed toward the intended detector or sample rather than allowing positioning errors to reduce transmission. When alignment remains stable, the setup can maintain stronger signal quality and more consistent timing conditions. These improvements support better temporal resolution and make kinetic measurements more reliable across the experiment.
A typical adjustment sequence positions the optical components, then uses the mirrors or retroreflectors to keep the beam collinear and maximize transmitted intensity. The optical path length is subsequently varied when the experiment requires a different delay. These adjustments coordinate beam direction, signal strength, and timing rather than treating them as independent settings.
In pump-probe spectroscopy, alignment supports controlled timing between an excitation pulse and a later measurement. Varying the optical path length changes when the probe samples the photoexcited system, while stable beam positioning helps preserve transmitted intensity. The resulting measurements can follow molecular dynamics over the selected delays after excitation.
Aligned time-resolved experiments can monitor how a chemical system changes after photoexcitation. In particular, time-resolved absorption measurements use controlled excitation-to-measurement timing to generate kinetic information. Stable alignment improves the reliability of those measurements, helping researchers evaluate molecular dynamics through changes recorded at different delays.