Alignment of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina

Shichu Chang; Wenjin Xu; Weijia Fan; John A. McDaniel; Marta Grannonico; David A. Miller; Mingna Liu; Hao F. Zhang; Xiaorong Liu

doi:10.3791/65237

Journal

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Neuroscience

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Alignment of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Alignment of Visible-Light Optical Coherence Tomography Fibergrams with Confocal Images of the Same Mouse Retina

1,425 Views

•

07:02 min

•

June 30, 2023

DOI:

10.3791/65237-v

DOI:

10.3791/65237-v

•

07:02 min

June 30, 2023

•

5 Views

Shichu Chang¹, Wenjin Xu¹, Weijia Fan², John A. McDaniel¹, Marta Grannonico¹, David A. Miller², Mingna Liu¹, Hao F. Zhang², Xiaorong Liu^1,3,4,5

¹Department of Biology,University of Virginia, ²Department of Biomedical Engineering,Northwestern University, ³Department of Ophthalmology,University of Virginia, ⁴Program in Fundamental Neuroscience,University of Virginia, ⁵Department of Psychology,University of Virginia

Transcript

We present a protocol for aligning in vivo VIS-OCT images with ex vivo immunostained retinal nerve fibers to validate our novel imaging technology. VIS-OCT enables visualization of retinal nerve fibers in vivo, and this process confirms the accuracy of our findings. Our protocol combines in vivo and ex vivo imaging to provide a real time visualization of retina structure and confirm findings.

The process is applicable to mouse models of eye diseases, provided the anterior eye portion allows for optical imaging. This includes diabetic retinopathy and retinal ischemia models. These studies lay the foundation for establishing an objective evaluation of neuro damage in humans, which can significantly improve glaucoma diagnosis and treatment in the future.

Summary

The present protocol outlines the steps for aligning in vivo visible-light optical coherence tomography fibergraphy (vis-OCTF) images with ex vivo confocal images of the same mouse retina for the purpose of verifying the observed retinal ganglion cell axon bundle morphology in the in vivo images.