Embryonic Fillet Preparation
Embryonic Fillet Preparation - Related Videos
03:16
Larval Fillet Preparation: A Method to Visualize Intact Sensory Neurons and Associated Epidermal Cells
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2023
This video describes how to prepare larval fillets used to visualize sensory dendritic arborization (da) neurons and their associated epidermal cells. The featured protocol shows in detail how to remove muscle tissue from the body wall while preserving the cells' morphology, which improves immunostaining and visualization of the otherwise obscured da neurons and epidermal cells.
06:41
Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
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Cited by 13 •
2016
Studies of neuronal morphogenesis using Drosophila larval dendritic arborization (da) neurons benefit from in situ visualization of neuronal and epidermal proteins by immunofluorescence. We describe a procedure that improves immunofluorescence analysis of da neurons and surrounding epidermal cells by removing muscle tissue from the larval body wall.
09:38
Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells
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Cited by 170 •
2012
The quality of mouse embryonic fibroblasts (MEFs) is dictated by the right strain of mouse such as CF-1. Pluripotency-supportive MEFs and conditioned media (CM) obtained from these should contain optimal concentrations of Activin A, Gremlin and Tgfβ1 needed for the Activin/Nodal and FGF pathways to co-operatively maintain self-renewal and pluripotency.
10:51
A System for ex vivo Culturing of Embryonic Pancreas
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Cited by 23 •
2012
Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.
09:23
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
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Cited by 7 •
2009
We describe a process for fixation, embedding, sectioning, and imaging of late stage Drosophila embryos for Trasmission Electron Microscopy of the embryonic heart tube. This technique allows for the visualization of the heart tube lumen as well as the basement membrane, which lines the lumen of the heart.
FAQs
Precise cuts open the embryo while maintaining the relative arrangement of its developing tissues. Spreading the specimen then presents internal structures in a broad, accessible plane rather than leaving them enclosed within the intact embryo. This organization helps researchers follow tissue boundaries and compare the positions of structures such as the neural tube, somites, and organ primordia.
Removing the surrounding yolk clears material that would otherwise cover the embryonic tissue during dissection and imaging. It also makes the embryo easier to open and spread without losing access to internal structures. As a result, researchers can examine tissue organization directly and prepare the specimen for downstream visualization or labeling procedures.
The preparation allows direct examination of structures including the neural tube, somites, and organ primordia. Their exposed arrangement supports anatomical mapping and analysis of how tissues are positioned relative to one another. Because these spatial relationships remain available for inspection, the method is useful for studying morphogenesis, the coordinated shaping and organization of developing tissues.
The workflow begins by removing the surrounding yolk, followed by making precise cuts along the embryo. The opened tissue is then spread into a flattened arrangement and secured for observation. Careful execution of these stages determines whether internal structures remain accessible and spatially organized, which directly affects the quality of subsequent microscopy, mapping, or staining.
Once the tissue has been spread and secured, its exposed surface supports high-resolution microscopy and immunostaining. Researchers can visualize anatomical features directly, label selected tissue components, and document their positions within the flattened specimen. The same preparation therefore combines structural observation with tissue-specific analysis, helping connect visible anatomy to developmental organization.
Researchers can apply the method to compare normally developing embryos with specimens carrying genetic or experimental perturbations. The preparation exposes comparable anatomical regions, allowing differences in tissue arrangement, organ primordia, or morphogenetic patterning to be examined directly. These comparisons help reveal how altered developmental conditions affect the organization of internal embryonic structures.