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A System for ex vivo Culturing of Embryonic Pancreas
A System for <em>ex vivo</em> Culturing of Embryonic Pancreas
JoVE Journal
Biology
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JoVE Journal Biology
A System for ex vivo Culturing of Embryonic Pancreas

A System for ex vivo Culturing of Embryonic Pancreas

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10:51 min

August 27, 2012

DOI:

10:51 min
August 27, 2012

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Summary

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Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.

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