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A System for ex vivo Culturing of Embryonic Pancreas

A System for ex vivo Culturing of Embryonic Pancreas

Article DOI: 10.3791/3979-v 10:51 min August 27th, 2012
August 27th, 2012



Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.


Ex Vivo Culturing Embryonic Pancreas Pancreatic Organogenesis Early Pancreas Formation Diabetes Pancreatic Cancer Cell-replacement Therapies Embryogenesis Dorsal And Ventral Foregut Endoderm Epithelial Buds Proliferation Branching Differentiation Tissue Architecture Epithelial Remodeling Cell Positioning Branching Morphogenesis
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