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10:51 min
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August 27, 2012
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Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.
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Cite this Article
Petzold, K. M., Spagnoli, F. M. A System for ex vivo Culturing of Embryonic Pancreas. J. Vis. Exp. (66), e3979, doi:10.3791/3979 (2012).
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