Hot Water Extraction
Hot Water Extraction - Related Videos
09:48
Employing Pressurized Hot Water Extraction (PHWE) to Explore Natural Products Chemistry in the Undergraduate Laboratory
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Cited by 13 •
2018
Here, we employ a pressurized hot water extraction (PHWE) method, which utilizes an unmodified household espresso machine to introduce undergraduates to natural products chemistry in the laboratory. Two experiments are presented: PHWE of eugenol and acetyleugenol from cloves and PHWE of seselin and (+)-epoxysuberosin from the Australian plant Correa reflexa.
05:31
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
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Cited by 168 •
2012
We describe a modified hot aqueous-phenol extraction method for purifying lipopolysaccharide (LPS) from Gram-negative bacteria. Once extracted, the LPS can be subsequently analyzed by SDS-PAGE and visualized by direct staining or Western immunoblot.
06:57
Determining Viral Disinfection Efficacy of Hot Water Laundering
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2022
In response to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, a laboratory protocol was developed to test the viral disinfection efficacy of hot water laundering of cloth face coverings, cotton scrubs, and denim pants. The Phi6 virus (bacteriophage) was used as the organism to test disinfection efficacy.
10:13
Working with Hot and Cold Sources
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2023
Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA Working with extreme temperatures, both high and low, is an integral part of many laboratory operations. For many, mentioning a laboratory instantly evokes the mental picture of a Bunsen burner. Bunsen burners and hot plates are used extensively in small and large operations in research laboratories and industries, thus making it necessary for all users to be aware of their safe handling...
11:59
Extraction and Detection of Geosmin and 2-Methylisoborneol in Water and Fish using High-Capacity Sorptive Extraction Probes and GC-MS
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Cited by 1 •
2025
This protocol presents a fully automated workflow for the extraction of geosmin and 2-methylisoborneol from water and lipid-rich fish tissues. The method allows for the early detection of these molecules before they reach odor thresholds. Representative data from an aquaculture setting are provided.
FAQs
Heating can increase the solubility of compounds in water and promote molecular diffusion through the sample matrix. As a result, target substances may move more readily from the solid or complex material into the liquid phase. The temperature therefore affects how efficiently the extraction proceeds, while the appropriate outcome still depends on the compounds and matrix being studied.
Contact time determines how long compounds can transfer into the water, whereas mixing influences contact between the heated water and the sample. Adjusting these conditions can change how much of a target substance enters the aqueous phase. They are therefore important variables when comparing extracts or interpreting differences in apparent extraction efficiency.
Selectivity depends on both the sample matrix and the target compounds, so the aqueous extract may not represent every substance present in the material. The method is consequently most informative when researchers interpret results in relation to water-soluble chemicals and the specific environmental sample, rather than treating extraction as universally comprehensive.
A basic workflow brings a solid or complex sample into contact with heated water, allows sufficient contact time with mixing, and collects the resulting aqueous extract. The liquid phase can then be used for analysis of transferred compounds. These stages connect operational conditions with the final information obtained about water-soluble substances in the sample.
The approach can be applied to soils, sediments, and other complex environmental materials when the research goal includes water-soluble chemicals. It can help characterize contamination by transferring relevant compounds into an aqueous extract. The material and target substances should guide interpretation because extraction selectivity varies with both.
In environmental research, the resulting extract can support both contamination characterization and evaluation of treatment or recovery processes. Using water instead of organic solvents may also reduce hazardous solvent waste. These benefits make the method relevant where researchers need aqueous sample preparation while still considering whether the target compounds transfer efficiently.