This protocol presents a fully automated workflow for the extraction of geosmin and 2-methylisoborneol from water and lipid-rich fish tissues. The method allows for the early detection of these molecules before they reach odor thresholds. Representative data from an aquaculture setting are provided.
Harrington, R. J., Cole, R., Szafnauer, R., Mayser, J. P., Pearson, M., Noufi, C., Burr, G., Peterson, B. C. Extraction and Detection of Geosmin and 2-Methylisoborneol in Water and Fish using High-Capacity Sorptive Extraction Probes and GC-MS. J. Vis. Exp. (221), e67280, doi:10.3791/67280 (2025).