Genome Folding
Genome Folding - Related Videos
03:00
Molecular Chaperones and Protein Folding
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2020
The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein. The...
08:31
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
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Cited by 2 •
2013
Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.
01:49
Protein Folding
0 Views •
2020
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation which is critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.Protein Structure Is Critical to Its Biological FunctionProteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
01:49
Protein Folding
0 Views •
2026
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation which is critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.Protein Structure Is Critical to Its Biological FunctionProteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
19:50
Interview: Protein Folding and Studies of Neurodegenerative Diseases
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Cited by 2 •
2008
In this interview, Dr. Lindquist describes relationships between protein folding, prion diseases and neurodegenerative disorders. The problem of the protein folding is at the core of the modern biology. In addition to their traditional biochemical functions, proteins can mediate transfer of biological information and therefore can be considered a genetic material. This recently discovered function of proteins has important implications for studies of human disorders. Dr. Lindquist also...
FAQs
Nucleosomes package DNA by wrapping it around histone proteins, creating a chromatin framework that affects whether genomic regions can be accessed. Because accessibility is tied to which genes are active, changes in this packaging can alter regulatory control without changing the DNA sequence itself. This makes nucleosome organization a key mechanistic link between chromatin structure and gene activity.
Cohesin and CTCF are proteins that help shape contacts among folded chromatin regions. Their role is important because genome folding depends not only on DNA and histones but also on proteins that organize larger-scale interactions. Examining these factors helps researchers relate changes in nuclear architecture to altered regulatory contacts and, consequently, to differences in gene activity.
These structural features organize the genome at different interaction scales. Loops can bring distant regulatory elements near promoters, domains organize groups of chromatin interactions, and active or inactive compartments distinguish broader nuclear environments. Considering all three helps researchers interpret how spatial arrangement influences regulatory access and why changes in nuclear architecture may accompany changes in cellular gene activity.
Chromosome conformation capture methods reveal structural changes in genome folding that may accompany mutations or altered cellular states. By comparing organization between conditions, researchers can investigate whether changes in nuclear architecture coincide with differences in regulatory interactions or gene activity. The resulting structural information complements genetic analysis by showing how sequence changes or cellular context relate to chromosome organization.
Comparing different cellular states can show whether nuclear architecture changes together with gene regulation. This approach is especially relevant when cells differ in developmental condition or when a genetic alteration is present. Researchers can use the comparison to connect altered folding patterns with changes in regulatory contacts and identify structural features associated with a particular cellular state.
Genome folding connects genetic information with the spatial organization that helps regulate gene activity. In developmental research, comparing folding patterns can clarify how nuclear architecture relates to changing cellular states. In disease-focused studies, researchers can examine whether mutations or altered conditions coincide with structural changes, extending genetic analysis by considering both DNA changes and their effects on chromosome organization.