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JoVE Journal
Immunology and Infection

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Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
 

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Article DOI: 10.3791/3899-v 08:31 min May 26th, 2013
May 26th, 2013

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Summary

Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.

Tags

Isolation Genome Analysis Single Virions Single Virus Genomics Whole Genome Amplification Sequencing Single Microbial Cells Genomic Characterization Cultivation Viruses Ubiquitous Numerous Entities Environments Isolating Characterizing Genomes Flow Cytometry High Molecular Weight Genomic DNA
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