Sonication Procedure
Sonication Procedure - Related Videos
07:24
Sonication Extraction of Lipid Biomarkers from Sediment
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
09:05
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
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2015
Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.
10:35
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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Cited by 16 •
2017
Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.
03:22
Low Frequency Ultrasound Application: A Method to Determine the Effect of Sonication on Human Leukemia Cells
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2023
This video describes the selective damage of human leukemia cells by using low frequency ultrasound. This application of low frequency ultrasound in a preclinical in vitro condition demonstrates that cell destruction can be attained in order to examine and compare the effects of sonication on leukemia and normal cells.
10:10
Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ
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Cited by 5 •
2014
Immunostaining is an effective technique for visualizing specific cell types and proteins within tissues. By utilizing sonication, the protocol described here alleviates the need to dissect Drosophila melanogaster tissues from late-stage embryos and larvae before immunostaining. We provide an efficient methodology for the immunostaining of formaldehyde-fixed whole mount larvae.
FAQs
Acoustic cavitation is central to sonication: sound energy creates cavitation, and the resulting mechanical forces generate shear within the sample. Those forces can disrupt cells and tissues or break DNA and chromatin into smaller fragments. In genetic workflows, controlling this physical action matters because fragment size affects whether material is suitable for later genomic, regulatory, or sequencing analyses.
Power, pulse duration, temperature, and sample volume are the main controllable conditions identified for sonication. Adjusting them allows researchers to regulate the extent of disruption while limiting heat-related damage. This balance is important when preparing genomic DNA, fragmenting chromatin, or generating material for sequencing libraries, because each application depends on controlled treatment rather than indiscriminate breakdown.
An ultrasonic probe and an ultrasonic bath are two ways to transfer acoustic energy to a genetic sample. The overview supports their use for disrupting cells, tissues, DNA, or chromatin, but does not assign identical performance to each. The relevant choice is therefore the available energy-delivery format and the controlled conditions required for the intended downstream analysis.
A practical workflow is to place the biological material in an ultrasonic probe or bath, select power and pulse duration, account for sample volume, and control temperature during treatment. The resulting material can then move into a downstream genetic workflow. Keeping these conditions controlled helps produce disruption or fragmentation appropriate to the planned analysis.
Sonication supports several genetics workflows, including genomic DNA preparation, chromatin fragmentation for immunoprecipitation, and library preparation for sequencing. In each case, acoustic treatment prepares biological material for a subsequent analysis rather than serving as the final measurement. Its value lies in making cells, tissues, DNA, or chromatin suitable for these downstream procedures.
Material processed through sonication can support downstream investigation of gene structure, gene regulation, and genome-wide variation. Fragmented DNA or chromatin provides input for workflows that examine these features, while library preparation connects the treatment to sequencing analysis. Thus, the procedure contributes an upstream sample-preparation step to broader genomic and regulatory studies.