Transgenic Reporter Mice
Transgenic Reporter Mice - Related Videos
08:41
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
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Cited by 6 •
2021
This protocol describes the dynamics of viral infections using luciferase- and fluorescence-expressing recombinant (r)SARS-CoV-2 and an in vivo imaging systems (IVIS) in K18 hACE2 transgenic mice to overcome the need of secondary approaches required to study SARS-CoV-2 infections in vivo.
08:51
Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic
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Cited by 14 •
2012
Described here is a technique in which lipopolysaccharide is injected into the lactating mouse mammary gland via the nipple to simulate mastitis, a condition commonly caused by bacterial infection. Lipopolysaccharide injection results in increased nuclear factor kappa B (NF-κB) signaling, visualized through bioluminescent imaging of an NF-κB luciferase reporter mouse.
09:32
Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils
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Cited by 1 •
2017
Peripheral injection of alpha-synuclein fibrils into the peritoneum or tongue of Tg(M83+/-:Gfap-luc+/-) mice, which express human alpha-synuclein with the familial A53T mutation and firefly luciferase, can induce neuropathology, including neuroinflammation, in their central nervous system.
07:30
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
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Cited by 7 •
2016
This article describes how to inject viral vectors into the mouse frontal cortex to test behavioral assays that require GPCR heteromeric formation.
07:48
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
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Cited by 15 •
2015
In this article we describe the principles for designing and performing a multi-color lineage tracing experiment using R26R-Confetti mice. We provide a specific protocol for tracking corneal epithelial cells which can be modified for other tissues of interest.
FAQs
Regulatory DNA acts as the control element that links the selected gene’s expression to reporter output. When that regulatory sequence is active in particular cells, during a defined developmental stage, or under a physiological condition, the linked signal identifies that context. This makes the model useful for comparing where and when gene regulation occurs in living tissues.
The reporter molecule determines how gene activity is observed or measured. Fluorescent proteins provide a visible signal that can reveal expression in selected tissues, whereas an enzyme such as luciferase supports measurement of reporter activity. Choosing between these reporter types therefore depends on whether the study emphasizes visualizing spatial patterns or quantifying a biological response.
A reporter signal can show whether the regulatory program associated with a selected gene is active in different cells, developmental stages, or physiological states. Comparing these contexts helps distinguish tissue-specific expression from changes associated with development or altered physiology. The resulting pattern connects genetic regulation with observable activity inside the animal rather than in an isolated sample.
Tissue-specific output helps researchers determine which cells or tissues engage a selected genetic program. This spatial information can separate localized activity from expression occurring throughout the animal, making it valuable for studying gene function and signaling pathways. Interpreting the signal in its tissue context also supports comparisons among developmental stages or physiological conditions.
A typical design starts by choosing the gene or regulatory program to investigate, then placing its relevant regulatory DNA in control of a reporter gene. The resulting genetically engineered mice are examined for reporter output in living tissues. Researchers can then compare signals across cells, developmental stages, physiological conditions, or experimental treatments to relate regulation to biological context.
These models are well suited to questions about where a gene is active, when its activity changes, and how that pattern relates to gene function. They can map tissue-specific expression, follow cell lineages, and monitor signaling pathways. Because observations occur in living tissues, the approach also supports investigation of dynamic biological processes rather than only fixed expression patterns.
Reporter activity can mark cells associated with a selected genetic program, allowing researchers to examine their distribution in tissues and across developmental contexts. Following the resulting pattern provides information about how labeled cell populations relate to development or tissue organization. In genetics, this connects regulatory activity with the location and progression of cells within the animal.
Reporter output can be compared before, during, or after a disease-related or treatment-related condition to identify changes in selected gene activity. Such comparisons may reveal altered signaling pathways or shifts in tissue-specific regulation. The models therefore provide an in vivo way to connect a physiological response with the genetic program associated with that response.