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Chapters
- 00:05Title
- 01:24Preparing Transgenic Mice
- 02:08Intraductal Injection of LPS
- 05:20Bioluminescent Imaging to Visualize NF-kB Activity
- 06:29Image Analysis
- 07:27Practice Injection Results and IVIS Images
- 08:29Conclusion
Described here is a technique in which lipopolysaccharide is injected into the lactating mouse mammary gland via the nipple to simulate mastitis, a condition commonly caused by bacterial infection. Lipopolysaccharide injection results in increased nuclear factor kappa B (NF-κB) signaling, visualized through bioluminescent imaging of an NF-κB luciferase reporter mouse.
Tags
Intraductal InjectionLPSMouse ModelMastitisNF-κB ReporterTransgenic AnimalDisease ProgressionPre-clinical ModelsLipopolysaccharideLactating Mouse Mammary GlandSimulated InfectionInflammationNuclear Factor Kappa BBioluminescent ImagingLuciferase Reporter MouseRednessSwellingImmune Cell InfiltrationStraight-forward MethodInsulin SyringeMammary Duct
