Calcified Disc
Calcified Disc - Related Videos
09:12
Calcified Artery Preparation and Processing with Preserved Morphology and RNA for Digital Spatial Profiling
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2026
This protocol includes a step-by-step workflow for calcified vascular specimens: tissue handling, decalcification, RNA validation, calcification level detection, and region-of-interest selection strategies on the Nanostring GeoMx Digital Spatial Profiler (DSP). The goal is to present a comprehensive method for preserving vascular tissue morphology and RNA for reliable spatial transcriptomic analysis.
14:33
Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
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Cited by 13 •
2012
The generation, purification and cell invasion of intracellular, cytoplasmic full length DISC1 protein aggresomes from cell cultures and of a labeled, multimeric recombinant DISC1 protein fragment in E. coli are described. Cell invasiveness is shown for recipient cells in cell culture and for neurons in vivo after stereotactical brain inoculation.
02:52
Calcification Assay to Measure Calcium Concentration in Aortic Valve Interstitial Cells
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2025
This video demonstrates an assay technique for assessing the calcification of the aortic valve interstitial cells in vitro. The assay helps to quantify the calcium concentration using the arsenazo III reagent, a calcium-sensitive dye that reacts with calcium ions to form a purple-colored calcium-arsenazo complex.
07:29
Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis
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Cited by 32 •
2010
Undecalcified bone histology provides important information for a variety of clinical and research applications. It is technically challenging, particularly with large size specimens. This video illustrates the process of producing good quality sections and demonstrates the technical difficulties and methods with which to overcome them.
08:55
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
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Cited by 16 •
2017
This protocol describes the isolation, culture, and calcification of rat-derived valve interstitial cells, a highly physiological in vitro model of calcific aortic valve disease (CAVD). Exploitation of this rat model facilitates CAVD research in exploring the cell and molecular mechanisms that underlie this complex pathological process.
FAQs
Location and extent help determine how a calcified disc may affect spinal structure and surrounding tissues. A small, limited finding may have different significance from more extensive mineralized material, particularly when symptoms or nerve irritation are present. Clinicians therefore interpret the distribution and size of the abnormality alongside pain, stiffness, inflammation, degeneration, and neurological findings rather than relying on the image alone.
Mineralized material can appear as increased density on radiographic imaging, making the finding identifiable during spine evaluation. X-rays, computed tomography, and magnetic resonance imaging may contribute different views of the spinal abnormality, but the appearance must be considered with the patient’s symptoms and examination. Imaging supports clinical interpretation by showing structural changes that may or may not explain the complaints.
Symptoms depend on more than the presence of calcium deposits. The disc’s location, the extent of structural alteration, associated inflammation or degeneration, and possible nerve irritation all influence clinical impact. Consequently, one patient may experience pain or stiffness while another has an imaging finding without comparable symptoms. This variability is why imaging results require correlation with the clinical picture.
Evaluation combines the patient’s reported symptoms, neurological examination, and spinal imaging. Symptoms help identify pain, stiffness, or possible nerve-related complaints, while the examination assesses neurological effects. X-rays, computed tomography, or magnetic resonance imaging can demonstrate associated structural findings. Considering these sources together helps clinicians judge whether the disc abnormality is relevant to the patient’s presentation.
These imaging approaches are relevant when clinicians need to assess spinal structural changes associated with a calcified disc. Radiographic imaging can show increased density from mineralized material, while computed tomography and magnetic resonance imaging may be included in the broader evaluation. The choice and interpretation depend on the clinical situation, the suspected location, and the need to relate imaging to symptoms.
Recognition of the finding helps clinicians distinguish disc-related structural changes from other potential causes of back or neck symptoms. Its clinical significance is judged using location, extent, associated inflammation or degeneration, symptoms, and neurological findings. That integrated assessment can inform decisions about monitoring or treatment, while avoiding conclusions based only on the presence of calcification on an image.