Collection Techniques
Collection Techniques - Related Videos
09:39
Technique to Collect Fungiform (Taste) Papillae from Human Tongue
0 Views •
Cited by 30 •
2010
Knowledge of molecular mechanisms underlying gustatory transduction has recently enjoyed significant advances, largely due to using animal models. However, the wide diversity in taste sensitivity and specificity among mammals warrants studies in human tissue. We describe a biopsy technique to collect living taste cells from the papillae on human tongue.
08:08
A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
0 Views •
Cited by 272 •
2008
Transgenic (Tg) mouse models of AD provide an excellent opportunity to investigate how and why Aβ or tau levels in CSF change as the disease progresses in human patients. Here, we demonstrate a refined cisterna magna puncture technique for serial CSF sampling from the mouse.
11:06
Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques
0 Views •
Cited by 16 •
2017
Here, we describe a minimally invasive laparoscopic technique for serial sampling of liver and mesenteric lymph nodes (MLN) in macaques that allows for increased sampling frequency, and reduces the potential for surgical complications when compared to performing a laparotomy.
03:08
Protein Lysate Extraction: A Technique to Lyse Organoids to Collect Cellular Proteins
0 Views •
2023
In this video, we demonstrate the extraction of protein lysates from prostate organoids cultured in a basement membrane matrix ring. Analysis of the extracted protein fractions can help study the lineage marker expression in the organoids.
05:22
Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling
0 Views •
Cited by 1 •
2019
Here, we present a protocol for collecting the floral fragrance volatiles from blooming flowers, using a non-destructive sampling procedure.
FAQs
Specimen selection determines whether the collected material can support the intended laboratory assessment or clinical investigation. Blood, urine, tissue, and other biological materials may provide different types of information, so the choice must match the diagnostic, monitoring, treatment-related, or research purpose. An unsuitable specimen can reduce the reliability of subsequent testing even when handling is otherwise consistent.
Aseptic handling limits contamination during collection and subsequent management of the specimen. This matters because contaminants can alter the sample’s characteristics, complicate laboratory processing, and undermine the reliability of results. Consistent aseptic practice also reduces avoidable risks to patients and healthcare personnel, making it an important quality and safety component rather than merely a procedural preference.
The container must be suitable for the specimen and the intended testing process. Its purpose is not only to hold the material, but also to help preserve the specimen’s relevant characteristics during handling and transport. Using an unsuitable container can contribute to sample deterioration or processing problems, which may weaken diagnostic, monitoring, or research reliability.
Accurate labeling and appropriate transport are essential after a specimen has been obtained. Labeling connects the material with the correct patient and clinical purpose, while transport conditions help preserve its characteristics before processing. Together, these steps reduce avoidable identification and handling errors and support dependable laboratory work for diagnosis, monitoring, treatment decisions, and research.
Transport should maintain the characteristics that make a specimen useful for its intended analysis. Because collection may involve blood, urine, tissue, or other biological material, the conditions used after collection must be compatible with the specimen and its testing purpose. Poorly controlled transport can introduce processing errors or reduce the reliability of information obtained from the sample.
In medicine, these techniques support more than a single laboratory result. Collected specimens and clinical information can contribute to diagnosis, follow changes in disease or health status, inform treatment decisions, and support investigations of biological processes. Consistency across collection, labeling, handling, and transport helps make the resulting information more dependable for patient care and research.