Murine Intestine
Murine Intestine - Related Videos
08:45
Tissue Engineering of the Intestine in a Murine Model
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Cited by 29 •
2012
This article and the accompanying video present our protocol for generating tissue-engineered intestine in the mouse, using an organoid units-on-scaffold approach.
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
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Cited by 52 •
2016
Here we describe the detailed procedure of intestinal ischemia-reperfusion in mice which results in reproducible injury without mortality to encourage the standardization of this technique across the field. This model of intestinal ischemia-reperfusion injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.
08:14
Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
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Cited by 55 •
2016
There is growing interest in the quantitative characterization of intestinal lymphocytes owing to increasing recognition that these cells play a critical role in a variety of intestinal and systemic diseases. In this protocol, we describe how to isolate single cell populations from different small-intestinal compartments for subsequent flow cytometric characterization.
07:05
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
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Cited by 2 •
2017
Target-specific probes represent an innovative tool for analyzing molecular mechanisms, such as protein expression in various types of disease (e.g., inflammation, infection, and tumorigenesis). In this study, we describe a quantitative three-dimensional tomographic assessment of intestinal macrophage infiltration in a murine model of colitis using F4/80-specific fluorescence-mediated tomography.
FAQs
Barrier studies focus on epithelial cells and their tight junctions, which regulate movement across the intestinal lining. When permeability changes, researchers can examine how that may alter exposure of underlying immune and host cells to luminal contents. This makes the murine intestine useful for connecting epithelial barrier behavior with intestinal homeostasis and inflammation.
Microbial interactions add a biological variable beyond the intestinal tissue itself. Intestinal microbes communicate with immune and host cells, influencing tissue homeostasis and inflammation. In a murine intestine experiment, examining this relationship can help researchers determine whether an observed response reflects epithelial activity, immune regulation, microbial influence, or an interaction among these factors.
Mouse findings require careful interpretation because mouse and human intestinal physiology are not identical. A response observed in the murine intestine may clarify a disease mechanism or indicate a possible therapeutic direction, but it does not by itself establish that the same response, treatment effect, or safety profile will occur in patients.
Researchers can use murine intestinal models to examine digestion, barrier function, immunity, disease processes, and responses to drugs. The choice of readout depends on the research question: investigators may focus on nutrient absorption, epithelial permeability, immune activity, inflammation, microbial interactions, or treatment response. Comparing these outcomes helps link tissue-level changes to broader disease biology.
Inflammatory bowel disease, infection, and cancer are major medical contexts for this system. The models can help investigate how intestinal tissues respond during disease and how candidate interventions alter those responses. Their value lies in connecting mechanisms, such as changes in permeability or inflammation, with disease-relevant outcomes, while retaining the need for cautious translation.
Studies of microbiome-host interactions use the intestine as a setting where microbial signals, epithelial barriers, immune cells, and host tissues can be considered together. This integrated view is relevant when a medical question concerns homeostasis or inflammation rather than a single cell type. It can also inform investigation of disease mechanisms and drug responses.