Nirf Imager
Nirf Imager - Related Videos
09:43
In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis
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Cited by 32 •
2011
We detail a new near-infrared fluorescence (NIRF) catheter for 2-dimensional intravascular molecular imaging of plaque biology in vivo. The NIRF catheter can visualize key biological processes such as inflammation by reporting on the presence of plaque-avid activatable and targeted NIR fluorochromes. The catheter utilizes clinical engineering and power requirements and is targeted for application in human coronary arteries. The following research study describes a multimodal imaging strategy...
08:09
Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
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Cited by 24 •
2015
This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.
10:12
Near-infrared Fluorescence Imaging of Abdominal Aortic Aneurysms
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2023
Source: Arvin H. Soepriatna1, Kelsey A. Bullens2, and Craig J. Goergen1 1 Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 2 Department of Biochemistry, Purdue University, West Lafayette, Indiana Near-infrared fluorescence (NIRF) imaging is an exciting optical technique that utilizes fluorescent probes to visualize complex biomolecular assemblies in tissues. NIRF imaging has many advantages over conventional imaging methods for noninvasive imaging of diseases.
01:45
Visualization of Mouse Cerebral Thrombi Using Near-Infrared Fluorescence Imaging
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2025
Source: Kim, D. E., et al. Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli. J. Vis. Exp. (2016)This video demonstrates near-infrared fluorescent (NIRF) imaging to visualize thrombi in excised brain tissue labeled with a fibrin-specific probe. Near-infrared light excites the probe, causing the thrombus to fluoresce, enabling high-contrast thrombi visualization in the major cerebral artery and small cortical blood vessels.
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
FAQs
The system directs near-infrared light toward the target and detects fluorescence emitted at longer wavelengths. A sensitive camera records this signal and generates contrast between fluorescent regions and surrounding tissue. Because the output is produced during illumination and detection, clinicians and researchers can obtain spatially localized information in real time during procedures or experimental imaging.
Near-infrared light can penetrate several millimeters into tissue and is less affected by visible-light background than many conventional visual observations. These properties help the imager reveal fluorescent structures beneath or within tissue while preserving spatial information. The resulting view does not replace anatomical imaging, but it can complement methods that provide broader structural context.
Administered fluorescent agents create a detectable signal that identifies selected tissues, structures, or regions during imaging. Their fluorescence allows the camera to distinguish the labeled target from surrounding tissue, improving localization during a clinical procedure or research observation. This approach is especially relevant when the structure of interest is difficult to identify from ordinary visual anatomy alone.
A typical workflow includes positioning the imager over the area of interest, illuminating the target with near-infrared light, and collecting emitted fluorescence with the system’s sensitive camera. The displayed signal provides real-time spatial contrast for interpretation during the procedure. When a fluorescent agent has been administered, the detected pattern can help localize labeled tissue or structures.
The technique is useful when a procedure requires real-time localization of fluorescently visible anatomy. Surgical guidance can use the contrast to identify labeled structures or lesions, while lymphatic mapping can use fluorescence to follow or locate lymphatic regions. In both settings, the imager adds spatial information during the intervention and complements conventional anatomical imaging.
Fluorescence patterns can provide information about regions relevant to tissue perfusion assessment or the location of fluorescently labeled lesions. The camera displays these signals as spatial contrast against nearby tissue, allowing observations during clinical procedures or research. Its value lies in adding real-time fluorescence information to anatomical views rather than serving as a standalone replacement for them.