Nt-probnp
Nt-probnp - Related Videos
13:34
Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
0 Views •
Cited by 20 •
2012
A step by step protocol to isolating and identifying RNA associated complexes through RIP-Chip.
11:00
Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes
0 Views •
Cited by 18 •
2017
Intracellular calcium recycling plays a critical role in regulation of systolic and diastolic function in cardiomyocytes. Here, we describe a protocol to evaluate sarcoplasmic reticulum Ca2+ reserve and diastolic calcium removal function in cardiomyocytes by a calcium imaging system.
06:18
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
0 Views •
Cited by 19 •
2018
Current multiplexed diagnostics to detect Zika, chikungunya, and dengue viruses require complex sample preparation and expensive instrumentation, and are difficult to use in low resource environments. We show a diagnostic that uses isothermal amplification with target-specific strand displaceable probes to detect and differentiate these viruses with high sensitivity and specificity.
11:32
Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
0 Views •
Cited by 23 •
2009
Regulated endocytosis governs the cell surface expression levels of the majority of membrane proteins. Here we utilize reducible, membrane impermeant biotinylation reagents to measure the endocytic rate of the dopamine transporter (DAT), a polytopic membrane protein. The method facilitates a straightforward approach to measuring the endocytic rate of most plasma membrane proteins.
11:08
Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
0 Views •
Cited by 10 •
2011
We demonstrate how to dissect and culture chick E4 statoacoustic ganglion and E6 spinal cord explants. Explants are cultured under serum-free conditions in 3D collagen gels for 24 hours. Neurite responsiveness is tested with growth factor-supplemented medium and with protein-coated beads.
FAQs
Ventricular muscle cells release proBNP when pressure or volume increases. Cleavage then separates the precursor into BNP, which is biologically active, and NT-proBNP, which is inactive. Because NT-proBNP remains in the circulation, an immunoassay can quantify it as a measurable signal of the cardiac stress associated with this cellular response.
Age and kidney function can influence the concentration measured in blood, so the same numerical result may not carry identical interpretive meaning in every patient. Clinicians should consider these factors alongside symptoms, examination findings, and imaging rather than treating the biomarker value as a standalone conclusion. This contextual approach helps prevent overinterpretation of a laboratory result.
BNP and NT-proBNP arise from the same proBNP cleavage event but are not identical analytes. BNP is the biologically active product, whereas NT-proBNP is the inactive N-terminal fragment that remains in circulation and is measured by immunoassay. Recognizing this distinction clarifies why a result labeled NT-proBNP represents a specific molecular component, not BNP itself.
Clinicians obtain a blood sample and quantify the circulating fragment with an immunoassay. The laboratory value is then brought back into the clinical assessment, where it is considered with the patient’s symptoms, physical findings, and imaging. This workflow makes the test a supportive piece of evaluation rather than a substitute for broader assessment.
In suspected heart failure, clinicians use the concentration alongside symptoms, physical findings, and imaging to support diagnostic assessment. This combined approach links the laboratory measurement with the patient’s presentation and imaging findings. Its value is therefore greatest when it contributes to broader clinical judgment about whether heart failure is a plausible explanation for the findings.
Beyond diagnostic support, the result can help clinicians estimate disease severity and prognosis, then provide a biomarker measure for monitoring treatment response. These uses address different clinical questions: the first concerns the current or expected course of illness, while the second concerns change over time. Interpretation still depends on surrounding clinical information, including relevant patient factors.