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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Article DOI: 10.3791/3851-v • 13:34 min • September 29th, 2012
September 29th, 2012 •
1,2, 1,2, 2, 1,2, 2, 1,2,3
1Molecular Microbiology and Immunology, University of Missouri, 2Department of Surgery, University of Missouri, 3Child Health, University of Missouri

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Summary
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A step by step protocol to isolating and identifying RNA associated complexes through RIP-Chip.

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Isolation Identification MRNAs MicroRNAs Protein Components Ribonucleoprotein Complexes RIP-Chip High-throughput Sequencing Microarray Analysis Global Gene Expression Analysis Post-transcriptional Gene Regulation RNA Binding Proteins (RBP) MRNA Localization Stability Translation Ribonucleoprotein (RNP) Complex De Novo MRNA Targets Cellular Extracts Protein Output RNP Immunoprecipitation-microarray (RIP-Chip) Experimental Conditions Post-transcriptional Gene Regulation Mechanisms Ribonucleoprotein Interactions
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