Tumor-infiltrating Leukocytes
Tumor-infiltrating Leukocytes - Related Videos
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Cited by 15 •
2015
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
2011
A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
07:11
Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation
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Cited by 33 •
2016
This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.
02:02
A Three-Dimensional Co-culture Model of Infiltrating T Cells in Tumor Spheroids
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2025
This video demonstrates a method to establish a three-dimensional tumor spheroid and T cell co-culture. An agarose cast with microwells containing tumor spheroids is prepared, followed by the addition of T cells. After incubation, this setup allows studying the interaction of T cells with tumor spheroids in a controlled three-dimensional environment.
06:49
Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy
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Cited by 3 •
2017
Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45+ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.
FAQs
Their effect depends on functional state rather than presence alone. Some infiltrating cells can recognize tumor-associated antigens and participate in responses that damage malignant cells, whereas others may create immune suppression or promote tumor growth through their signaling and interactions. Assessing activity alongside cell abundance therefore gives a more meaningful view of their influence within the tumor microenvironment.
Antigen recognition helps immune cells distinguish tumor-associated targets, while cytokine signaling coordinates communication among leukocytes and surrounding cells. These processes can strengthen antitumor activity or contribute to suppression, depending on the cellular context. Cell-to-cell interactions add another layer of regulation, making the local signaling environment important when interpreting how infiltrates affect cancer progression.
T lymphocytes, B lymphocytes, natural killer cells, macrophages, and other leukocytes represent different components of the immune response within tumor tissue. Their relative presence can reveal the composition of the tumor immune environment, but composition alone does not establish function. Combining cellular identity with density and functional activity helps distinguish potentially protective responses from immune conditions associated with tumor support.
These three features provide complementary information. Composition shows which leukocyte populations are present, density indicates how extensively they accumulate in tumor tissue, and functional activity suggests whether they are responding effectively or participating in suppression. Together, the measurements help researchers characterize tumor immunity, investigate relationships with cancer progression, and evaluate immune features that may be relevant to patient outcomes.
Researchers examine the cellular makeup and behavior of infiltrates as indicators of the tumor microenvironment. Differences in composition, density, or activity can be compared with disease-related outcomes to explore whether particular immune patterns are associated with more favorable or less favorable courses. This information supports investigation of tumor immunity and can help identify immune features relevant to clinical interpretation.
Their presence and functional characteristics provide context for immunotherapy decisions. Immune checkpoint blockade is studied in relation to the existing tumor immune environment, while adoptive tumor-infiltrating lymphocyte therapy uses the relevance of tumor-associated lymphocytes as part of its therapeutic rationale. Profiling these cells can therefore connect tumor biology with strategies intended to strengthen or redirect antitumor immunity.