Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45+ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.
Männ, L., Klingberg, A., Gunzer, M., Hasenberg, M. Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy. J. Vis. Exp. (123), e55450, doi:10.3791/55450 (2017).