Plasmodium Berghei
Plasmodium Berghei - Related Videos
12:48
Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
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Cited by 11 •
2013
A method for isolation of adherent inflammatory leukocytes from brain blood vessels of Plasmodium berghei ANKA-infected mice is described. The method allows quantification as well as phenotypic characterization of isolated leukocytes after staining with fluorescent antibodies and subsequent analysis by flow cytometry.
10:16
Generating Genetically Modified Plasmodium berghei Sporozoites
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2023
Malaria is transmitted through inoculation of the sporozoite stage of Plasmodium by infected mosquitoes. Transgenic Plasmodium has allowed us to understand the biology of malaria better and has contributed directly to malaria vaccine development efforts. Here, we describe a streamlined methodology to generate transgenic Plasmodium berghei sporozoites.
07:14
Ookluc: A Plasmodium berghei Line for Identifying Transmission-blocking Compounds
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2025
Plasmodium sexual development occurs in the mosquito midgut. Targeting the parasite's sexual stages is a promising strategy to block transmission. We developed Ookluc, a transgenic P. berghei expressing nanoluciferase after zygote formation, enabling high-throughput screening of transmission-blocking compounds. This article details the methods for screening using Ookluc.
09:02
An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
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Cited by 17 •
2014
Tuberculosis and malaria are two of the most prevalent infections in humans and major causes of morbidity and mortality in impoverished populations in the tropics. We established an experimental model system to study outcome of malaria-tuberculosis coinfection in mice after challenge with both pathogens via their natural route of infection.
05:20
Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means
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Cited by 7 •
2013
The paramagnetic properties of hemozoin are used to isolate late stages of Plasmodium falciparum-infected red blood cells growing in culture. The method is simple and fast and does not affect the subsequent invasive capabilities of the parasites.
FAQs
Following the parasite across its liver and blood stages lets investigators distinguish effects associated with initial tissue development from those that emerge after red-blood-cell infection. That staged biology is valuable in Plasmodium berghei studies because neurological changes can be considered alongside infection progression, rather than treated as an isolated brain event. It supports stage-aware interpretation of malaria-related pathology.
Endothelial activation can alter the behavior of cells lining brain vessels, while blood-brain barrier dysfunction can weaken the separation between circulating factors and neural tissue. When these changes occur with neuroinflammation, they provide a mechanistic framework for examining how infection-related vascular and immune disturbances may contribute to brain injury during experimental cerebral malaria.
Assessing immune signaling together with neuronal function and behavior connects molecular and vascular events to consequences observable at the organism level. In neuroscience research, this combination helps determine whether systemic infection is associated only with altered brain vessels or also with functional changes. Plasmodium berghei therefore supports integrated analysis of inflammation, neural function, and behavior under controlled conditions.
The susceptible mouse context matters because infection can produce endothelial activation, blood-brain barrier dysfunction, and neuroinflammation in that setting. This gives investigators a defined experimental system for relating infection to neurological outcomes. It also helps establish whether observed brain-related changes occur within the broader course of malaria biology rather than independently of infection.
An experiment can begin with mosquito transmission, followed by the parasite's progression through liver cells and then red blood cells in the mouse. Investigators can then examine brain-related vascular, inflammatory, neuronal, or behavioral outcomes in relation to that progression. This sequence preserves the connection between infection stage and neurological findings.
Relevant outcomes span several levels: endothelial activation and blood-brain barrier dysfunction describe vascular changes; immune signaling and neuroinflammation indicate inflammatory responses; neuronal function and behavior capture functional consequences. Examining these categories together helps researchers connect systemic infection with brain effects and assess whether an intervention limits neurological damage.
Because infection can be studied under controlled experimental conditions, the model provides a framework for testing therapies aimed at limiting neurological damage. Investigators can relate treatment effects to brain-vessel status, barrier function, inflammatory signaling, neuronal function, or behavior. This approach helps assess whether an intervention reduces infection-associated brain injury alongside its effects on malaria biology.