Stereotaxic Injection
Stereotaxic Injection - Related Videos
04:17
Stereotaxic Viral Injection for Targeted Neuronal Gene Editing in the Mouse Brain
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2025
Source: Fricano-Kugler, C. J., et al. Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection. J. Vis. Exp. (2016)This video demonstrates stereotaxic viral injection for precise neuronal gene editing in the mouse brain. The protocol ensures accurate virus delivery to the dentate gyrus, enabling fluorescent labeling and CRISPR-mediated gene knockout to study neural circuits, gene function, and disease mechanisms in vivo.
03:58
Stereotaxic Injection of Viral Vectors for Glial Cell Reprogramming in a Mouse Model
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2025
Source: Pereira, M. et. al., In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors. J. Vis. Exp. (2019)This video demonstrates stereotaxic injection of an adeno-associated virus into the cerebral cortex of a transgenic mouse to deliver Cre-dependent neuronal reprogramming and fluorescent reporter genes into glial cells.
11:28
Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
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Cited by 25 •
2016
The CRISPR/Cas9 system offers the potential to make targeted genome editing accessible and affordable to the scientific community. This protocol is intended to demonstrate how to create viruses that will knockout a gene of interest using the CRISPR/Cas9 system, and then inject them stereotaxically into the adult mouse brain.
04:12
Stereotaxic Injection of Viruses for Stable Genetic Modification in a Mouse Model
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2025
Source: Porlan, E., et al. Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects. J. Vis. Exp. (2016)This video demonstrates stable genetic modification of neural stem cells (NSCs) in the ventricular-subventricular zone (V-SVZ) through stereotaxic injection of a virus-carrying therapeutic gene in a mouse model. The virus enters NSCs, undergoes reverse transcription, integrates into the genome, and...
08:39
Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
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Cited by 18 •
2013
Viral vectors allow for targeted gene manipulation. We demonstrate a method for conditional gene expression or ablation in the mouse spinal cord, using stereotaxic injection of a viral vector into the dorsal horn, a prominent site of synaptic contact between primary somatosensory afferents and neurons of the central nervous system.
FAQs
The coordinate system specifies a target’s position in three dimensions, allowing the operator to align the stereotaxic frame with a selected brain or spinal cord region. Controlled depth and trajectory then guide the needle or micropipette through tissue toward that location. This spatial planning helps restrict delivery to the intended neural structure rather than distributing the substance broadly.
Trajectory and depth determine which tissue the needle or micropipette reaches before a substance is administered. Small changes in either variable can shift delivery away from the intended region, reducing spatial specificity. Controlling both parameters is therefore essential when researchers want to connect a localized manipulation with changes in neural activity, gene expression, or behavior.
The reagent selected for Stereotaxic Injection shapes the experimental question. Drugs can be used to manipulate neural systems, viral vectors to investigate gene expression, and tracers to examine neural organization. Other reagents may support additional measurements. Matching the substance to the study goal allows researchers to examine circuit function, molecular changes, or behavioral consequences in a targeted location.
A stereotaxic frame provides the mechanical support for positioning the subject and guiding the delivery device. Researchers use three-dimensional anatomical coordinates to select the target, then control the needle or micropipette’s depth and trajectory during placement. These components work together to produce reproducible, localized delivery within the brain or spinal cord.
Neuroscientists use this method when a study requires manipulation or measurement in a defined neural region. Applications include investigating neural circuits, examining gene expression, tracing anatomical connections, and relating localized interventions to behavior. Its value is greatest when regional specificity matters for understanding brain function, disease mechanisms, or possible therapeutic strategies.
Targeted injection limits delivery to a selected brain or spinal cord location, whereas systemic administration does not provide the same regional precision. This difference helps researchers associate an observed molecular, circuit, or behavioral outcome with a particular neural site. The approach therefore supports more spatially specific tests of neural function and disease-related mechanisms.