/
/
Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
This content is Free Access.
Chapters
- 00:05Title
- 00:45Prepare 293FT/293GP Cells for Transfection
- 03:02Calcium Phosphate Transfection and Viral Particle Collection
- 05:24Concentration and Purification of the Virus
- 06:42Stereotaxic Injection
- 09:40Results
- 10:59Conclusion
The CRISPR/Cas9 system offers the potential to make targeted genome editing accessible and affordable to the scientific community. This protocol is intended to demonstrate how to create viruses that will knockout a gene of interest using the CRISPR/Cas9 system, and then inject them stereotaxically into the adult mouse brain.
