Suspension Culture
Suspension Culture - Related Videos
03:19
Generating an Organoid Culture from a Brain Tumor Cell Suspension
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2025
This video describes the preparation and cultivation of brain tumor organoids from single-cell suspension. Using a laminin-rich extracellular matrix as a scaffold and culture media for nutrients, cells are facilitated to multiply and self-organize within the organoid. Consequently, ex vivo organoids are generated, mimicking cellular diversity and the microenvironment of brain tumors.
10:17
The Tail Suspension Test
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Cited by 534 •
2012
The tail-suspension test is validated as an experimental procedure to assess antidepressant efficacy of drug treatments in mice. Mice are suspended by their tails for six minutes and escape-related behaviors are assessed. We describe procedures used in conducting the tail suspension test.
11:44
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
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Cited by 5 •
2013
Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully 15N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.
04:16
ECM Suspension Preparation: A Technique to Obtain Homogenous Suspension from Tissue-derived Extracellular Matrix
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2023
This video describes the preparation of a homogenized and digested extracellular matrix suspension from decellularized and lyophilized tissues. This technique helps in fabricating ECM-derived porous foams and microcarriers for use as substrates in advanced 3D cell culture models.
01:17
Colloids and Suspensions
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2023
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles visible to the naked eye or seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. The suspended particles in a suspension settle out after some time of mixing. The separation of particles from a suspension is...
FAQs
Removing the requirement for attachment allows these cells to remain within the liquid medium and form free-floating neurospheres. This arrangement provides a controlled setting for examining how neural populations survive, renew themselves, and later differentiate. Because cells can be maintained and expanded in a shared medium, researchers can study population-level responses under consistent culture conditions.
These growth factors are included in the culture medium to support neural stem and progenitor cell survival and to promote formation of free-floating neurospheres. Their presence helps maintain a population suitable for studying self-renewal and differentiation. Changing the experimental treatment while using this growth-factor-supported system can reveal how neural cells respond under defined conditions.
Uniform sampling makes comparisons across neural cell populations more consistent, while expansion provides enough material to examine behavior over a broader experimental scale. In neuroscience, this is useful when investigators compare self-renewal, differentiation, or treatment responses in neural stem and progenitor cells. The approach therefore connects controlled cell-based observations with studies of development, disease, and regeneration.
An appropriate liquid medium, nonadherent conditions, and growth factors such as EGF and FGF form the core setup. Neural stem and progenitor cells are maintained within this environment as free-floating neurospheres. This combination supports survival and continued study of cell behavior, while keeping the culture aligned with experiments on self-renewal, differentiation, or treatment responses.
Researchers may select it when they need to expand neural stem or progenitor cells, obtain more uniform sampling, or examine cellular responses in a controlled liquid environment. The method is especially relevant to questions about neural development, disease, and regenerative research, where self-renewal and differentiation are important experimental outcomes.
Findings from these cultures can relate neural self-renewal, differentiation, and treatment responses to larger questions in development, disease, and regenerative research. The system also supports controlled expansion and more uniform sampling, helping researchers compare cell populations while focusing on cellular mechanisms. Its value lies in linking experimentally observed behavior with neuroscience models and treatments.