Zebrafish Interneuromast Cells
Zebrafish Interneuromast Cells - Related Videos
02:39
Monitoring Recovery of Laser-Ablated Interneuromast Cells in Zebrafish Using Confocal Microscopy
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2025
Source: Volpe, B. A., et al. Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells. J. Vis. Exp. (2020) This video demonstrates monitoring interneuromast cell recovery in zebrafish using confocal microscopy, tracking cell death and repair through time-lapse fluorescence imaging.
11:17
Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells
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Cited by 13 •
2020
Laser ablation is a widely applicable technology for studying regeneration in biological systems. The presented protocol describes use of a standard laser-scanning confocal microscope for laser ablation and subsequent time-lapse imaging of regenerating interneuromast cells in the zebrafish lateral line.
06:55
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
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Cited by 42 •
2011
A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.
09:22
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
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Cited by 44 •
2017
Here, we describe xenograft zebrafish models using two different injection sites, i.e., perivitelline space and duct of Cuvier, to investigate the invasive behavior and to assess the intravasation and extravasation potential of human breast cancer cells, respectively.
08:01
Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish
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Cited by 35 •
2011
Limiting dilution cell transplantation assays are used to determine the frequency of tumor-propagating cells. This protocol describes a method for generating syngeneic zebrafish that develop fluorescently-labeled leukemia and details how to isolate and transplant these leukemia cells at limiting dilution into the peritoneal cavity of adult zebrafish.
FAQs
Wnt and Fgf pathways help regulate whether interneuromast cells are maintained, proliferate, or differentiate. Their activity therefore connects local signaling conditions with the production of new sensory-organ components. Studying these pathways allows researchers to examine how developmental signals control progenitor behavior and how changes in signaling may alter the formation of intercalary neuromasts.
Neighboring neuromasts provide local signals that help coordinate interneuromast-cell maintenance and development as the lateral-line system expands. This spatial relationship is important because progenitor activity must remain organized between existing sensory organs. Examining these interactions helps reveal how nearby tissues influence progenitor decisions rather than treating cell proliferation and differentiation as isolated events.
Differentiation can produce intercalary neuromasts that contain mechanosensory hair cells and supporting cells. This outcome links progenitor behavior to the cellular organization required for sensory detection. Following the transition from an epithelial progenitor population to a structured neuromast helps researchers study how vertebrate sensory organs acquire their specialized cell types.
Hair-cell injury provides a context for examining how the lateral-line system responds when sensory components are damaged. Because the zebrafish lateral line is accessible and can be observed during this response, researchers can investigate whether progenitor maintenance, proliferation, and differentiation contribute to repair. The resulting observations connect developmental mechanisms with sensory-organ regeneration.
Researchers can use live imaging to follow interneuromast cells within the accessible zebrafish lateral line over developmental or injury-related responses. This approach supports direct observation of cell behavior, including changes associated with migration, proliferation, and differentiation. The ability to monitor these events in vivo makes the system useful for linking cellular dynamics with sensory-organ formation and repair.
These cells provide a tractable model for investigating how vertebrate mechanosensory systems are assembled and repaired. Their relationship to neuromasts enables studies of sensory-organ development, cell migration, stem-cell behavior, and regeneration within the lateral line. Findings from this model can provide scientific context for understanding principles relevant to regenerative neuroscience.