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Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells
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Chapters
- 00:04Introduction
- 00:42Larva Mounting
- 02:16Prospective Target Location
- 04:02Pre-Ablation Imaging
- 05:49Laser Ablation
- 07:03Post-Ablation Imaging and Regeneration Time-Lapse Microscopy
- 08:25Results: Representative Interneuromast Cell (INMC) Ablation Imaging and Gap Measurement
- 10:23Conclusion
Laser ablation is a widely applicable technology for studying regeneration in biological systems. The presented protocol describes use of a standard laser-scanning confocal microscope for laser ablation and subsequent time-lapse imaging of regenerating interneuromast cells in the zebrafish lateral line.
