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JoVE Journal
Developmental Biology

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Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells
 

Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells

Article DOI: 10.3791/60966-v
May 20th, 2020

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Summary May 20th, 2020

Laser ablation is a widely applicable technology for studying regeneration in biological systems. The presented protocol describes use of a standard laser-scanning confocal microscope for laser ablation and subsequent time-lapse imaging of regenerating interneuromast cells in the zebrafish lateral line.

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