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Immunology and Infection

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JoVE Journal Immunology and Infection
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
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Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

Article DOI: 10.3791/1420-v • 11:18 min • November 3rd, 2009
November 3rd, 2009 •
1, 2, 3, 2,4
1Dynamac, Inc., 2Department of Biological Sciences, University of Cincinnati, McMicken College of Arts and Science, 3Animal Parasitic Disease Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 4National Exposure Research Laboratory, US Environmental Protection Agency

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This study describes the development of a modified CsCl method that easily purifies T. gondii oocysts from feces of infected cats that are suitable for molecular biological and tissue culture manipulation

Tags

Toxoplasma Gondii Oocysts Cesium Chloride Gradient Protozoan Pathogen Food-borne Disease Outbreaks Feline Species Sexually Replication Infectious Oocyst Tissue Cysts Contaminated Meat Contaminated Soil Contaminated Water Latent Infection Toxoplasmic Encephalitis Immunocompromised Seroprevalence Waterborne Outbreaks
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