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Neuroscience

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JoVE Journal Neuroscience
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Mushjärnberikad immunpelletsplattfärgningsmetod för histonkvantifiering

Mouse Brain Histone Modification Quantification Using Flow Cytometry

Article DOI: 10.3791/200981-v • 01:15 min • September 15th, 2023
September 15th, 2023 •
1, 2, 1
1Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health, University of British Columbia, 2Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health, University of British Columbia

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Kvantifiering globala histonposttranslationella modifieringar intranukleär flödescytometri isolerade mushjärnmikroglia genuttryckskontroll kromatinstruktur posttranslationella modifieringar histonsvansar acetylering lysinrester DNA-interaktioner tillgång till transkriptionsfaktor bromodomäninnehållande transkriptionsaktivatorer förbättrat genuttryck dynamisk reglering celldifferentiering cellulära miljöer stimuli nästa generations sekvenseringsmetoder hög Genomströmning kvantitativt mått
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