Encyclopédie des expériences JoVE
Microbiologie
0 vues • 2:52 min • July 1st, 2026
Seed a bacterial culture onto a filter paper placed on agar.
Take the test nanostructured material in a plate and add a medium to its surface.
Invert the filter paper onto the material, ensuring bacterial contact with the nanostructured surface.
Use an inert surface with a bacteria-seeded filter paper as the control.
Add a buffer to both plates to maintain humidity, and incubate.
Surface nanostructures impose stress and disrupt bacterial membranes, causing leakage of intracellular contents.
Additionally, metal ions released from the nanostructures generate reactive oxygen species and damage cellular components, reducing bacterial viability.
Transfer the filter papers to tubes containing the buffer.
Agitate vigorously and sonicate to release adhered bacteria. Collect the suspensions and perform serial dilutions.
Plate the dilutions onto agar, incubate for colony formation, and count the colonies.
Fewer colonies in the test compared to the control indicate the antibacterial activity of the nanostructured surface.
Prepare sterilized nitrocellulose papers by trimming them into 1 centimeter a diameter. Place the cut nitrocellulose paper onto an agar plate containing the appropriate medium.
Next, ad
Consultez la transcription complète et accédez à des milliers de vidéos scientifiques