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Microbiologie
0 vues • 4:15 min • July 1st, 2026
Begin by co-culturing amoebae with mutant strains derived from a single virulent bacterial isolate.
The amoeba engulfs the bacteria, mimicking the phagocytic activity of host immune cells.
Discard the medium and wash with fresh medium to remove extracellular bacteria.
Add a medium containing antibiotics and incubate it to kill the remaining extracellular bacteria.
Wash with fresh medium, then add medium supplemented with antibiotics. Next, add heat-killed feeder bacteria, then incubate.
The amoeba engulfs the feeder bacteria for nourishment.
During incubation, virulent mutant strains survive and divide within the amoeba.
Next, add a detergent solution and incubate to lyse the amoeba cell, releasing intracellular bacteria.
Spot the lysate onto agar medium, dilute with sterile water, and incubate.
Colonies derived from mutant bacterial strains appear on the agar surface.
Variation in colony counts among mutant bacterial strains indicates differential virulence in intracellular survival and replication within the amoeba.
Begin by culturing amoeba Acanthamoeba castellani, or AC, in 30 milliliters of Peptone yeast Glucose medium in 75 square centimeter tissue culture flasks at room temperature. Af
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