Immunofluorescence-Based Detection of Viruses Captured with Carbon Nanotube Arrays
Encyclopédie des expériences JoVE
Microbiologie
0 vues • 1:23 min • October 29th, 2026
Begin with a microfluidic device containing an inlet and an outlet connected to a microchip composed of aligned carbon nanotube arrays.
These arrays are made of carbon nanotubes with a specific intertubular distance to trap the virus.
Take a sample containing the avian influenza virus and flush it through the arrays.
As the sample flows through, the viruses are captured within the intertubular space between the nanotubes.
Flush with a buffer to remove any free viruses and cellular debris.
Take out the microchip and add primary antibodies.
Incubate to allow binding of the primary antibodies specifically to the surface proteins of the virus.
Wash with buffer to remove unbound antibodies.
Introduce fluorophore-tagged secondary antibodies and incubate.
The secondary antibodies attach to the primary antibodies.
Flush with buffer to remove unbound antibodies.
Under a fluorescence microscope, visualize the trapped viruses within the carbon nanotube arrays.
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