JoVE Journal
Biology
Biology
Un abonnement à JoVE est nécessaire pour afficher ce contenu.
Chapitres
résumé
We developed a quantitative DNA-binding, ELISA-based assay to measure transcription factor interactions with DNA. High specificity for the RUNX2 protein was achieved with a consensus DNA-recognition oligonucleotide and specific monoclonal antibody. Colorimetric detection with an enzyme-coupled antibody substrate reaction was monitored in real time.